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《Chinese Journal of Analytieal Chemistry》 2002-09
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Determination of Oxalic Acid in Plant Tissue and Root Exudate by Reversed Phase High Performance Liquid Chromatography

Yu Le 1, Peng Xinxiang 1, Yang Chong 2, Liu Yonghai 1, Fan Yanping 1 (Laboratory of Molecualr Plant Physiology, College of Biotechnology 1, Testing Center 2, South China Agricultural University, Guangzhou 510642)  
An improved method was described for the determination of oxalic acid in plant samples by reversed-phase high performance liquid chromatography. A reversed-phase C 18 column, an ultraviolet detector at 220 nm, and a mobile phase containing 0.5% KH 2PO 4 and 0.5 mmol/L tetrabutylammonium hydrogen sulfate (pH 2.0) were applied to the system, which allowed a good separation of oxalic acid from nitrate and other organic acids. The method was further tested for the analysis of the samples of soybean leaves and the root exudate, showing it is precise, sensitive and highly reproducible. The detection limit is 1.43 ng, relative standard deviation is 2.8% ( n=21). Recovery is around 100%. In addition, the collecting method of root exudate was improved to avoid loss of oxalic acid during the preparation, by which it is much easier to monitor the oxalic acid exuded from the non-oxalic acid accumulating plants.
【Fund】: 国家自然科学基金 (No .30 0 70 453);; 教育部博士点基金 (No .19990 0 5 0 0 2 )资助项目
【CateGory Index】: O657.7
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