Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Analytieal Chemistry》 2002-12
Add to Favorite Get Latest Update

Studies on the Assisted Actions of Furfuryl Alcohol for the Renaturation Efficiency of α-Chymotrysin by Liquid Chromatography

Shen Yehua, Wang Haibo, Wei Yinmo, Geng Xindu * (Institute of Modern Separation Science, Key Laboratory of Modern Separation Science in Shaanxi Province, Northwest University, Xi′an 710069)  
Using high performance hydrophobic interaction chromatography (HPHIC), the assisted actions of furfuryl alcohol in a guanidine hydrochloride (GuHCl) solution or on silica surface bonding ligands with the end group of furfuryl alcohol to the renaturation efficiency of α-chymotrysin (α-Chy) were investigated. The magnitudes of bioactive recovery of α-Chy were employed to characterize the contributions of furfuryl alcohol to α-Chy refolding in the two circumstances. By comparison of the results from size exclusion chromatogrpahy (SEC) HPHIC it may understand the different magnitudes in renaturation efficiency of furfuryl alcohol for both of solution and stationary phase, and the mechanism of the assisted actions of furfuryl alcohol may be attributed to form a complex between α-Chy and furfuryl alcohol either in solution, or on the surface of the HPHIC stationary phase. Furthermore, the optimazation of the amount of furfuryl alcohol in the GuHCl solution was also carried out.
【Fund】: 国家自然科学基金资助项目 (No .2 9675 0 17 39880 0 0 3)
【CateGory Index】: O657.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved