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Analysis of Lycopene by Supercritical Fluid Chromatography

Qi Guopeng, Zhao Suoqi  (The Key State Laboratory of Heavy Oil Processing, University of Petroleum of Beijing, Beijing 102249)  
Lycopene was separated by using supercritical fluid chromatography on a C 18 column with supercritical CO 2 as mobile phase, under the conditions of temperature in the range of 25~50℃, pressure 15.0 MPa~20.0 MPa, and concentration of the modifier ethanol in 0~20% and hexane in 0~30%. The retention time of lycopene is within 3 min. The lycopene extracted by supercritical propane, the feed and the extract residue from tomato skins were quantified accurately. The relative standard deviations were below 6%.
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