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《Chinese Journal of Analytical Chemistry》 2003-03
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Application for Multiwall Carbon Nanotubes Modified Electrode to Liquid Chromatography for Determination of the Thiols in Human Whole Blood

Lin Li, Cao Xuni, Zhang Wen, Zhou Yuyan, Li Jinhua, Jin Litong * (Department of Chemistry, East China Normal University, Shanghai 200062)  
A new chemically modified electrode(CME) was fabricated based on the immobilization of multi-wall carbon nanotubes fuctionalized with carboxylic group. The results indicated that the CME exhibited efficiently electrocatalytic oxidation for L-cysteine and glutathione with relatively high sensitivity, stability and long-life. Coupled with high performance liquid chromatography, the CME is utilized for electrochemical detection of thiols. The peak currents of L-cysteine and glutathione were linear to their concentrations ranged from 3.0×10 -7 mol/L to 1.0×10 -3 mol/L with the calculated detection limits of 1.2×10 -7 mol/L, 2.2×10 -7 mol/L, respectively. The method had been successfully applied to assess the contents of L-cysteine and glutathione in human whole blood.
【Fund】: 国家自然科学基金 (No .2 175 0 0 6);; 上海市纳米专项基金 (No .0 114nm0 72 )资助课题
【CateGory Index】: O657.7
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