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《Chinese Journal of Analytical Chemistry》 2003-05
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Isolation and Purification of α-Amanitin by Reversed Phase High Performance Liquid Chromatograph

Cao Fuxiang ( Biochemistry Laboratory, Central South Forestry University, Zhuzhou 412006) Zhang Zhiguang, Liang Songping ( College of Life Science , Hunan Normal University, Changsha 410081)  
α-Amanitin from Amanita juliginea is isolated and purified by using reversed phase high performance liquid chromatography under the condition of 0.02 mol/L aqueous ammonium acetate-acetonitrile mobile phase with gradient elution mode. The yield of α-amanitin may reach 6556 μg/g( dry-weight) .The purities of the product are over 99% . The recovery of the product is over 96% . The linear regressive equation of quantitative analysis for α-amanitin is given.
【Fund】: 国家科技部重点资助项目(96-C02-03-07)
【CateGory Index】: O657.72
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