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《Chinese Journal of Analytical Chemistry》 2004-02
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Determination of Caffeine by Micro High Performance Liquid Chromatography with A Molecularly Imprinted Capillary Monolithic Column

Yan Liushui, Wang Zonghua, Luo Guoan *, Wang Yiming ( Department of Chemistry, Tsinghua University, Beijing 100084 )  
A new micro high performance liquid chromatographic(μ-HPLC)method for highly selective separation and determination of caffeine was described. In this method, a molecularly imprinted capillary monolithic column prepared by in situ polymerization inside a ultraviolet transparent fused silica capillary using caffeine as template molecule was used. Several factors affecting the column performances in the preparation procedure were investigated and the chromatographic separation conditions were optimized. The results show that the obtained columns exhibit high resolution of 2.57 for separation of caffeine and its structural analogues. It has been applied to the determination of caffeine in greentea beverage, pepsi-cola and pharmaceutical tablets with satisfactory results.
【Fund】: 国家自然科学基金重大项目 (No .2 0 2 990 3 0 6)资助
【CateGory Index】: O657.7
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