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《Chinese Journal of Analytical Chemistry》 2004-03
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Determination of Phosphate by Fluorescence Quenching of Tb ~(3+)-Tiron Complex

Shi Lifang, Wang Dongyuan, Zhao Yibing* (Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005)  
A sensitive method by fluorescence quenching for the determination of inorganic phosphate is proposed. The method is based on the ability of phosphate to inhibit the formation of a strong fluorescent complex of Tb 3+ with Tiron. The optimal conditions for the determination of phosphates were studied, and the possibilities of spectrofluorimetric measurements of these systems were studied under optimal conditions (pH 6.9 in hexamethylene tetramine-HCl buffer, 1.2×10 -6 mol/L of Tb 3+, 4.0×10 -6 mol/L of Tiron, λ ex=317 nm, λ em=546 nm). The linear range of the determination was 0.005~30 mg/L and the detection limit was 4.1 μg/L for phosphate. The method has been applied to determine inorganic phosphate in urine samples with relative standard deviation of 3.5% and recovery of 93%~98%。
【CateGory Index】: O657.3
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2 LIU Lin-feng,LIU Zhong-fang,LIU Shao-pu,DUAN Hui School of Chemistry and Chemical Engineering,Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,Southwest University,Chongqing 400715,China;Fading Spectrophotometric Determination of Two Coenzyme I with Victoria Blue 4R[J];Journal of Southwest University(Natural Science Edition);2009-01
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