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《Chinese Journal of Analytical Chemistry》 2004-03
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Determination of Phosphate by Fluorescence Quenching of Tb ~(3+)-Tiron Complex

Shi Lifang, Wang Dongyuan, Zhao Yibing* (Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005)  
A sensitive method by fluorescence quenching for the determination of inorganic phosphate is proposed. The method is based on the ability of phosphate to inhibit the formation of a strong fluorescent complex of Tb 3+ with Tiron. The optimal conditions for the determination of phosphates were studied, and the possibilities of spectrofluorimetric measurements of these systems were studied under optimal conditions (pH 6.9 in hexamethylene tetramine-HCl buffer, 1.2×10 -6 mol/L of Tb 3+, 4.0×10 -6 mol/L of Tiron, λ ex=317 nm, λ em=546 nm). The linear range of the determination was 0.005~30 mg/L and the detection limit was 4.1 μg/L for phosphate. The method has been applied to determine inorganic phosphate in urine samples with relative standard deviation of 3.5% and recovery of 93%~98%。
【CateGory Index】: O657.3
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【References】
Chinese Journal Full-text Database 4 Hits
1 ZHENG Jie,LI Qi-zhong,ZHAO Yi-bing*(The Key Laboratory for Analytical Sciences of the Ministry of Education,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen,Fujian 361005);Determination of Coenzymes with Phosphates by Fluorescence Quenching of Tb~(3+)-Tiron Complex[J];Journal of Analytical Science;2007-02
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【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 Odeng Gaowa;Lian Xishan(Inner Mongolia University);Study on the Fluorescence of Terbium─Tifon─Octylphenyl Polyglycol Ether─polyacrylamie System[J];JOURNAL OF INNER MONGOLIA NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1994-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Du Xiao-wang (Dept.of Chemistry,Chongqing Normal University, Chongqing 400047,China);Preparation of cobalt phosphate Nanoparticles Under Microware Radiation[J];Journal of Chongqing Teachers College(Natural Science Edition);2004-01
2 Huang Zaifu ,Xu Wei,Guo Bin (Department of Environmental Science,Wuhan University,Wuhan 430072)Tang Yurong(Center of Instrumental Analysis, Wuhan University) Gan Zongren,Wang Yuncheng Wan Huifang(Wuhan General Hospital,Guangzhou Command) (Hospital of wuhan University);Determination of Total Calcium in Serum Using a New Calcium Ion-selective Electrode[J];Chinese Journal of Analytical Chemistry;1993-09
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【Secondary References】
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1 WANG Rui YU Zhen-Hong YU Ying(Institute of Communication,School of Communications and Control Engineering,Jiangnan University,Wuxi,Jiangsu 214122,P.R.China);Application of BP Neural Network Algorithm in the UV Absorption Spectrum of the Phosphate Solution[J];Chinese Journal of Spectroscopy Laboratory;2009-04
2 LIU Lin-feng,LIU Zhong-fang,LIU Shao-pu,DUAN Hui School of Chemistry and Chemical Engineering,Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,Southwest University,Chongqing 400715,China;Fading Spectrophotometric Determination of Two Coenzyme I with Victoria Blue 4R[J];Journal of Southwest University(Natural Science Edition);2009-01
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