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《Chinese Journal of Analytical Chemistry》 2004-05
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Study on the Interaction between Colchicine and Bovine Serum Albumins by Fluorescence Method

Liu Yongming *, Li Guizhi, Sun Xifen (College of Chemistry, Yantai University, Yantai 264005)  
The binding reaction between colchicine and bovine serum albumins (BSA) in aqueous solution was studied by fluorescence and ultraviolet-visibale absorption spectra. The results indicates that colchicine strongly binds to BSA with a molar ratio of 1∶1 and the equilibrium constant K 0 is 1.92×10 5 L/mol. The Stern-Volmer curve on the fluorescence of BSA quenched by series of colchicine concentrations shows good linear relationship, which illustrates the combination reaction of colchicine and BSA is a single static quenching process. The shortest binding distance ( r =3.63 nm) and energy transfer efficiency ( E =0.17) between donor (BSA) and acceptor (colchicine) were obtained by Frster's nonradiative energy transfer mechanism.
【CateGory Index】: O657.3
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