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《Chinese Journal of Analytical Chemistry》 2004-06
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Determination of Total Flavonoids in Llex Kudincha by Three Wavelength Spectrophotometry

Hou Dongyan *1,2 ,Hui Ruihua 1,2 , Mei Yang 2, Liu Xiaoyuan 3, Guan Chongxin 1 1(Department of Chemistry, Anshan Normal University , Anshan 114005) 2(Department of Chemistry, Liaoning Normal University, Dalian 116029) 3(Liaoning Longxing Biologe Science and Technology Co. Ltd., Anshan 114001)  
Determining the total flavonoids in Llex Kudincha by traditional spectrophotomeic method,baseline shift and asymmetric absorption peak were occurred on the absorption curve. The absorbance error of interfering components turbid solution ,Background changed with concentration changed and asymmetric absorption peak problems can be eliminated by three wavelength spectrophotomeic method. The flavonoids in Llex Kudincha with aluminate to produce stable complex which absorption there was red shift of longer wavelength . The regression equation of concentration ~Δ A was obtained:Δ A=-3.702×10 -3 +3.7023C ,correlation coefficient r =0.9994. The experimental results demostrate the total flavonoids concentrations in 600~1600 (mg/L) with Δ A obeying linear relation measured the absorbance at wavelength λ 1= 470 nm,λ 2=420 nm,λ 3=370 nm. The recovery is 98% ~112% and the coefficient of variation is 1.14%( n =9). The method is more advantageous than traditional spectrophotomeic method.
【Fund】: 辽宁省教育厅科学技术基金资助项目 (No .2 0 3 3 10 79)
【CateGory Index】: O657.3
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【References】
Chinese Journal Full-text Database 10 Hits
1 KUANG Chun-tao et al (College of Material Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004);Study on Extraction Process of Total Flavonoids from Pubescent Holly Root[J];Journal of Anhui Agricultural Sciences;2009-01
2 WANG Ke1,WANG Yong1,ZHAO Dong-bao1,3,LI Ming-jing1,LIU Xiu-hua1,2 and XU Ping-hui4(1.Environmental and Analytical Science Institute of College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004;2.Key Lab of Natural Drug and Immune Engineering,Henan Province,Kaifeng 475004;3.Jiyuan Vocational and Technical College,Jiyuan 454650;4.Xinxiang Biocaro biotech co.LTD,Xinxiang 453000);Determination of the contents of total flavonoids in lonicera japonica and its leaves by three wavelength spectrophotometry[J];Chinese Journal of Analysis Laboratory;2011-02
3 LI Na,MAO Yong-qiang(College of Science,Liaoning Engineering and Technology University,Fuxin,Liaoning 123000,China);Microwave Assisted Extraction Technology for Total Flavonoids from the Leaves of Llex kudincha[J];Guizhou Agricultural Sciences;2011-05
4 CHEN Cong-Jin HUANG Ke-Yinga LI Jiao-Juana WEI Long-Binb(Chemistry and Chemical Engineering College,Guangxi University,Nanning 530004,P.R.China)a(College of Material Science and Engineering,Central South University of Forestry and Technology,Zhuzhou,Hunan 412006,P.R.China)b(Guangxi Cadre School of Forestry,Nanning 530001,P.R.China);Research Advance on the Spectroanalysis Methods of Flavoniods in Plants[J];Chinese Journal of Spectroscopy Laboratory;2007-04
5 LIU Cheng-Lun Zhang Jing Xie Tai-Ping He Chun-Lan (College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400030,P.R.China);Research Progresses on Chromatographic and Spectral Analysis of Flavonoids Compounds[J];Chinese Journal of Spectroscopy Laboratory;2012-01
6 YU Chen-xiu;SUN Yi-min;WU Sheng;Anhui Traditional Chinese Medicine Professional School;Anhui Medicinal Plants Institute;Key Laboratory of Functional Molecular Solid of Anhui Province;College of Chemistry and Materials Science,Anhui Normal University;;Visual analysis of ultrasound wave assisted leaching process for extraction of sunscreening active ingredient in Ilex kudingcha leaves[J];China Surfactant Detergent & Cosmetics;2013-05
7 KUANG Chun-tao,LI Xiang-zhou,WANG Yu-xia,LIU Shen,WU Bin (College of Material Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China);Study on Determination Methods and Extraction Process of Total Flavonoids in Leaves of Ilex latifolia[J];Food Science;2009-06
8 YU Xiu-zhu,WANG Ling,DU Shuang-kui,LI Zhi-xi,ZHANG Jing-ya(College of Food Science and Engineering,Northwest A&F University,Yangling 712100,China);Optimization of Ultrasonic Extraction Process for Total Flavonoids from Hovenia Seeds by Response Surface Methodology[J];Food Science;2011-12
9 YU Chen-xiu1,2*,SUN Yi-min3,4,5,WANG Jun3,LU Feng3,ZHOU Dong-zi1 1Anhui Traditional Chinese Medicine Professional School,Wuhu 241000,China;2Anhui Medicinal plants Institute, Nanling 241314,China;3 Key Laboratory of Functional Molecular Solid of Anhui Province,Wuhu 241000,China; 4 College of Chemistry and Materials Science,Anhui Normal University,Wuhu 241000,China; 5Anhui Provincial Research Center of Extraction of Natural Product by Supercritical Fluid,Wuhu 241007,China;Visual Analysis of Extraction Process for Kudingcha Sunscreen Compositions[J];Natural Product Research and Development;2013-05
10 Feng Zhang, Gui-Wen Yang, Jin-Feng Zhang, Li-Guo An, Key Laboratory of Animal Resistance, College of Life Sciences, Shandong Normal University, Jinan 250014, Shandong Province, China;Inhibitory effects of Ginkgo biloba leaf flavonoids on proliferation of human gastric cancer cell line BGC823 in vitro[J];World Chinese Journal of Digestology;2005-21
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Li Meiqing 1, Sheng Xuan 2, Shao Xueguang *1 1(Department of Chemistry, University of Science and Technology of China, Hefei 230026) 2(Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230061);Separation and Determination of Active Components of Pueraria Lobata Isoflavonoid Extract by Reversed Phase High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2003-02
2 DING Ming yu,LI Qing yang (Department of Chemistry, Tsinghua University, Beijing 100084) and ZHAO Ji ping (Institute of Chemical Defence, Beijing 102205);Study on determination methods of total flavonoids in the extractive of hypericum perforatum L[J];Analytical Laboratory;2001-06
3 HUI Ruihua and HOU DongyanLiaoning University, 110036 Shenyang;QUANTITATIVE DETERMINATION OF RIFAMPICINUM BY THREE WAVELENGTH SPECTROPHOTOMETRY[J];SPECTROSCOPY AND SPECTRAL ANALYSIS;1995-05
4 Du Wei;Determination of Content of Total Flavonoids in Cili and Resources Development Prospect[J];Food Science;2003-01
5 Zhao Huichun,Feng Ruiqin,Fang Xin (Department of Chemistry,Beijing Normal University, Beijing, 100875);Fluorimetric Determination of Puerarin in “Yufongninxin”Tablets[J];JOURNAL OF INSTRUMENTAL ANALYSIS;1997-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Yan,CHEN Jian (Department of Chemistry,Hanshan Normol University,Chaozhou 521041,China);Ethanol Extraction Processing of Flavonoids from Herba Andrographitis[J];Anhui Chemical Industry;2009-02
2 ZHANG Jian-quan,ZHANG Wen-cheng,LIU Ling,ZHENG Ren-juan,ZHANG Hai-tao,WANG Wu(Hefei University of Technology,Hefei 230009,China);Research of the Solubitity of Oleanolic Acid in Supercritical CO_2[J];Anhui Chemical Industry;2009-06
3 TIAN Guo-zheng et al (The School of Biological Science and Technology, Enshi, Hubei 445000);Analysis and the Assessment of the Nutrition of Dipsacus Asper Wall in Different Growing Stages[J];Journal of Anhui Agricultural Sciences;2005-02
4 SUN Han-ju et al(College of Biological and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009);Study on the Technology of Tea Melon Seeds[J];Journal of Anhui Agricultural Sciences;2005-12
5 ZHANG Ying-cai et al (School of Life Science,Ningxia University,Yinchuan,Ningxia 750021);Summary of Researches on Anthraquinone Compounds of Rheum Tanguticum Maxim.et Bal. Var. Liupanshanense Cheng et Kao[J];Journal of Anhui Agricultural Sciences;2007-01
6 YAO Xiao-hui (Department of Life Science,Shangqiu Normal College,Shangqiu,Henan 476000);Medicinal Efficacy of Plantain and Measuring Method of Valid Composition[J];Journal of Anhui Agricultural Sciences;2007-04
7 LI Yu-ping et al(College of Life Science,Northwest A & F University,Yangling,Shaanxi 712100);Detection of Total Flavonoids in Cell Culture Substance of Carpesium.macrocephalum Franch.et Sav[J];Journal of Anhui Agricultural Sciences;2007-29
8 LI Feng et al(College of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010);Study on Extraction Technology of Water-soluble Crude Polysaccharide from Dioscorea opposita Thunb.cv.Tiegun[J];Journal of Anhui Agricultural Sciences;2008-11
9 SUN Xu-dong et al (Jiangxi Agricultural University,Nanchang,Jiangxi 330045);Study on Comprehensive Utilization of Functional Material in Citrus Pericarp[J];Journal of Anhui Agricultural Sciences;2008-19
10 ZHOU Jian-qing et al(Department of Chemistry,Qinghai Normal University,Xining,Qinghai 810008);Extraction and Content Determination of Flavonids in Aloe by Orthogonal Experiment[J];Journal of Anhui Agricultural Sciences;2008-20
China Proceedings of conference Full-text Database 10 Hits
1 WANG Wen-ming(Guangdong Food and Drug Vocational College,Guangzhou 510520,China.);Determination of Contents of Pachymic Acid in Poriae cutis by HPLC[A];[C];2012
2 ZHANG Lingyun,TAN Yaosen (Department of Tea Science,South China Agricultural University,Guangzhou 510642,China);A Study on the Effects of Fermentation Condition on Quality of Dancong Tea Wine[A];[C];2010
3 LI Hong-jian, GU Da-jian, SUN Shu-juan, SU Le-qun ,HUANG Xin (Shandong Province Qianfoshan Hospital, Jinan 250014, China);Study on dissolution of tetrahydropalmatine in Yuanhu Zhitong Dispersible Tablets in vitro[A];[C];2005
4 HUANG Xin, SU Le-quo, SUN Fu-jun, LI Li, MU Yan, LI Gui-hai ( Shandong Province Qianfoshan Hospital. Jinan 250014, China; Traditional Chinese Medicine Institute Shandong Province, Jinan 250014, China; Medical and Industty Institute Shandong Province, Jinan 250100, China);Study on pharmacokinetics of Yuanhu Zhilong Dispenible Tablets in dogs[A];[C];2006
5 (Northwest Institute of Nuclear Technology,Xi' an 710024) Tu Guo-rong Du Guang-xu Liu Xiang-feng Zhao Huan-zhen Wang Wu-shang Wang Xu-hui;Study of radiation impairment by ultraviolet ray and sun-screening Effectiveness of composite ultraviolet ray-absorption agent[A];[C];2005
6 ZHANG Jing-ze SHI Zuo-qing Department of Pharmacy, Medical College of Chinese People's Armed Police Forces, Tianjin 300162 The State Key Laboratory of Functional Polymeric Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University,Tianjin 300171;The study of purification of flavonoids from Hippohae rhamnoides L.on the coordination adsorption[A];[C];2006
7 Wang Li-juan Wang Min Wen Zhu (Tianjin Key Lab of Industrial Microbiology,College of Biotechnology,Tianjin University of Science and Technology,Tianjin,300457);Advances in studies on biotransformation of compounds of saponins in Chinese material medica[A];[C];2007
8 WANG Yue-e~(1,2),LI Xiao-jin,~(1,2*),WANG Guo-ping~2,JIA XIAO-guang~2,ZHU Jun~2 (1.Xinjiang Medical University,Urumqi 830054; 2.XinJiang Institute of Chinese traditional medical and ethical materia Medica,Urumqi,830002;);Preliminary test of chemical components for the seeds of Ferula sinkiangensisK[A];[C];2011
9 PEI Miaorong,XUAN Chunsheng,DUAN Xiujun,PEI Xiangping Department of Chinese Materia Medica,Shanxi University of Traditional Chinese Medicine, Taiyuan,030024;Chemical structure of complexes formed by acid and alkaline components of Pair Medicines in decoction[A];[C];2009
10 ;The Callus Culture of Ginkgo Biloba and Regulation of Its Metabolite[A];[C];1998
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 GAO Su-lian,WANG Xue-mei (Department of Chemistry of Anhui University, Hefei 230039,China)$$$$;Extraction and Determination of Triterpene and Flavonoid Constituents Isolated from the Glycyrrhiza[J];Journal of Anhui University(Natural Sciences);2000-04
2 LI Shui-qing et al(College of Chemical and Environmental Engineering,Yangtze University,Jingzhou,Hubei 434023);Absorption-photometric Determination of Total Flavonoids in A.graveolens[J];Journal of Anhui Agricultural Sciences;2007-09
3 GUO Xue-feng et al (International Centre for Bamboo and Rattan, SFA Key Open Laboratory on Bamboo and Rattan Science and Technology, Beijing 100102);Research Advances in Extraction, Isolation, Purification and Content Determination of Methods Flavonoids[J];Journal of Anhui Agricultural Sciences;2007-26
4 ZHONG Wei-ran et al (College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Study on the Cytochrome P450s in Phenylpropanoid Metabolic Pathway[J];Journal of Anhui Agricultural Sciences;2008-13
5 YU Ling et al(College of Horticulture and Landscape,Southwest University,Chongqing 400716);Mechanism and Application of RNA Interference[J];Journal of Anhui Agricultural Sciences;2009-07
6 FANG Yan-xi~1,ZHU Jin-kun~2(1.Department of Applied Biology,Anhui Science and Technalogy University,Fengyang 233100,China;2.Department of Fundamental Science,Anhui Science and Technalogy University,Fengyang 233100,China);Study on the Flavonoid Glycoside of Chrysanthemum[J];Journal of Anhui Science and Technology University;2006-02
7 GUAN Chong xin\ \ HUI Rui hua\ \ HOU Dong yan (Analytical and Testing Research,Anshan Teachers College,Liaoning Anshan 114005,China);Study on the Extraction of Flavonoids from the Scutellaria Baicalensys[J];Journal of Anshan Teachers College;2000-04
8 ZHANG Lan jie,ZHANG Wei hua,ZHENG Hong jun (Department of chemistry ,Anshan Normal University,Anshan Liaoning 114005,China);Determination of the total content of flavones in Qianshan Semen Cassiacs[J];Journal of Anshan Normal University;2003-04
9 ZHANG RoNG-QING HAN ZHENG-KANG CHEN JIE ZHANG CHONG-LI (Lab of Animal Physicly and Biochemistry, Nanjing Agric, Univ, Nanjing 210095) (Institute of Zoology, Chinese Academy of Sciences, Beijing 100080);EFFECTS OF DAIDZEIN ON MAMMARY GLAND DEVELOPMENT IN RATS[J];Acta Zoologica Sinica;1995-03
10 LI Ping WANG Yan hui MA Run yu (The Key Laboratory of Science and Technology of Controllable Chemical Reactions, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China);Antioxidation activities of polysaccharide PFCAⅢ from Fructus corni[J];Journal of Beijing University of Chemical Technology;2003-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 HAO Yan-zhi et al(School Hospital of Qingdao Agricultural University,Qingdao,Shandong 266109);Inhibitory Effects of Composite PSP on HeLa Cells of Human Cervical Cancer[J];Journal of Anhui Agricultural Sciences;2009-08
2 HUANG Hong-ying et al (Department of Chemistry and Life Science,Xiangnan University,Chenzhou,Hunan 423000);Study on Extraction Technological Conditions of Total Flavonoids from Toona sinensis Leaves[J];Journal of Anhui Agricultural Sciences;2010-11
3 YU Lin-hua,WANG Yi-qiang,LAN Hua,WANG Shuang (College of Life Science and Technology,Central South University of Forestry and Technology,Changsha,Hunan 410004);Research Progress on Pharmacological Functions of Folium Ginkgo or Extract of Ginkgo biloba[J];Northern Horticulture;2011-10
4 TU Baojun1, QIN Weidong2, ZHANG Long2, CHEN Qingchong2, SHI Weidong2 (1.Educational Administration Office, Xuzhou Institute of Technology, Xuzhou 221008; 2.College of Food Technology, Xuzhou Institute of Technology, Xuzhou 221008);Research Actualities and Development Strategies of Flavonoids in Asparagus[J];Journal of Changjiang Vegetables;2008-14
5 Chen Yingfeng,Liang Zhi(Department of Chemical Engineering,Maoming Vocational and Technical College,Maoming 525000,China);Optimization of Extraction Conditions of Flavonoids from Longan Seed[J];Guangdong Chemical Industry;2009-02
6 YIN Wen-qing,FENG Hua-fen,DUAN Shao-qing,TAN Qiang,CEN Ning-cai(State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources,College of Chemistry and Chemical Engineering,Guangxi Normal University,Guilin Guangxi 541004,China);Extraction of Total Triterpenoids from Ilex pubescens Hook leaves[J];Journal of Guangxi Normal University(Natural Science Edition);2012-02
7 Li Jiewen,Cai Guoxian,He Tingyu,Gu Wenxiang(College of Sciences,South China Agricultural University,Guangzhou 510642,China);Studies on the Determination Methods and Extraction Technology of Total Flavonoids from Myoporum Bontioides A. Gray[J];Guangdong Chemical Industry;2012-07
8 ZHU Zhen-yu,CAI Ya-mei and CHAI Yi-feng(School of Pharmacy,Second Military Medical University,Shanghai 200433);Pharmaceutical Analysis(Chinese Journals)[J];Chinese Journal of Analysis Laboratory;2012-10
9 SU Ben-wen, ZHAO Xi, HUANG Huai-sheng, ZHENG Hong-fa, YIN Xia, ZHONG Xing-gang, LIU Shu-juan (Tea Research Institute of Hunan Academy of Agricultural Sciences, Changsha 410125, China);Compared Study on the Extraction Method of Total Flavonoid From Green Tea and its Effect on Scavenging Hydroxyl Radicals[J];Tea Communication;2012-04
10 YANG Meng-meng,GUO Zhao-bin,YU Qun-li,LI Ru-ren(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China);Ultrasonic-assisted extraction of collagen[J];Journal of Gansu Agricultural University;2013-03
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Guo Nairong Luo Qingyao Chen Zhenhua Zeng Yun′e (Department of Chemistry,Wuhan University);The Principle and Application of Three-Wavelength Spectrophotometry[J];Chinese Journal of Analytical Chemistry;1983-06
2 Zhang Jintang Yao Benlin Liu Fuhuang Lu Xinchun(Department of Fine Chemical Technology,Wuhan Institute of Chemical Technology,Wuhan 430073,China);Determination of Flavonoids in the Extractive of Ginkgo Biloba Leaves with Ultraviolet Spectrophotometry[J];JOURNAL OF WUHAN INSTITUTE OF CHEMICAL TECHNOLOGY;1998-01
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