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《Chinese Journal of Analytical Chemistry》 2004-12
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Determination of Organic Acids in Fructus Mume by High Performance Liquid Chromatography

Guo Ying~1,2 , Xiao Zhaoping1, Wang Hong1, Huang Tianbao~*1 ~ 1 (Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041)~ 2 (Graduate School of Chinese Academy of Sciences, Beijing 100039)  
A reversed-phase high performance liquid chromatographic method was developed for determining malic acid, lactic acid, acetic acid, citric acid and succinic acid in Fructus mume on a Kromasil C_~18 column with 0.01 mol/L potassium dihydrogen phosphate buffer (pH 2.7) as mobile phase and ultraviolet detector at 215 nm. The column temperature was 17℃ and the flow rate was 0.5 mL/min. The results of the determination of organic acids in four kinds of Fructus mume were reported. The average recoveries of the method ranged from 98% to 105% with the relative standard deviation of 2.3%~7.8% and detection limit 10~75 ng (~S/N=3 ).
【Fund】: 四川省乐山市科技孵化基金资助项目(No.200204)
【CateGory Index】: O657.7
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