Preparation of Strychnine Molecularly Imprinted Monolithic Column
Zhang Jing, He Langchong *, Fu Qiang (College of Medicine, Xi′an Jiaotong University, Xi′an 710061)
Using strychnine as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate(EDMA) as the cross linker, a strychnine imprinted monolithic column was prepared for the separation of the strychnine and brucine by in situ molecular imprinted technique. Then, the synthesis condition was optimized. The result showed the best ratio of template, functional monomer and cross link was 1∶4∶16, and the toluene content in porogen was 18%. The effects of mobile phase composition, flow rate and column temperature on retention and separation factor were investigated. The maximum separation factor between strychnine and brucine was 3.5.