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《Chinese Journal of Analytical Chemistry》 2005-06
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Determination of Proline and Hydroxyproline in Human Urine with Capillary Electrophoresis-Electrochemiluminescence

Xue Jing, Liang Heng*, Li Tian, Wu Yayan (Key Laboratory of Biomedical Information Engineering of Ministry of Education, Separation Science Institute, Xi′an Jiaotong University, Xi′an 710049)  
A method of capillary electrophoresis (CE) with tris (2,2′-bipyridyl) ruthenium (Ⅱ) electrochemiluminescence (ECL) detection has been applied to the simultaneous determination of proline (Pro) and hydroxyproline (Hyp) in human urine. Under optimized conditions, such as detection potential at 1.18 V, electrokinetic injection at 10 kV for 10 s, seperation voltage at 20 kV, 10 mmol/L seperation buffer with pH 8.5, 5 mmol/L ru(bpy)~2+ _3 and 50 mmol/L phosphate buffer with pH 7.0 in the ECL cell, the linear ranges were 0.008~2 mmol/L for Pro with a correlation coefficient of 0.997, and 0.01~2 mmol/L for Hyp with a correlation efficient of 0.998. Detection limits for Pro and Hyp were 2 and 4 μmol/L respectively. When the method was applied to determine free and total Pro and Hyp in human urine, the ~recoveries were between 96.4% and 99.7%, respectively.
【Fund】: 国家自然科学基金资助项目(No.20299030 20175015)
【CateGory Index】: R446.12
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