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《Chinese Journal of Analytical Chemistry》 2005-06
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Determination of Proline and Hydroxyproline in Human Urine with Capillary Electrophoresis-Electrochemiluminescence

Xue Jing, Liang Heng*, Li Tian, Wu Yayan (Key Laboratory of Biomedical Information Engineering of Ministry of Education, Separation Science Institute, Xi′an Jiaotong University, Xi′an 710049)  
A method of capillary electrophoresis (CE) with tris (2,2′-bipyridyl) ruthenium (Ⅱ) electrochemiluminescence (ECL) detection has been applied to the simultaneous determination of proline (Pro) and hydroxyproline (Hyp) in human urine. Under optimized conditions, such as detection potential at 1.18 V, electrokinetic injection at 10 kV for 10 s, seperation voltage at 20 kV, 10 mmol/L seperation buffer with pH 8.5, 5 mmol/L ru(bpy)~2+ _3 and 50 mmol/L phosphate buffer with pH 7.0 in the ECL cell, the linear ranges were 0.008~2 mmol/L for Pro with a correlation coefficient of 0.997, and 0.01~2 mmol/L for Hyp with a correlation efficient of 0.998. Detection limits for Pro and Hyp were 2 and 4 μmol/L respectively. When the method was applied to determine free and total Pro and Hyp in human urine, the ~recoveries were between 96.4% and 99.7%, respectively.
【Fund】: 国家自然科学基金资助项目(No.20299030 20175015)
【CateGory Index】: R446.12
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【References】
Chinese Journal Full-text Database 10 Hits
1 XU Jin-ming1,TAO Hui-lin2(1 Department of Chemistry,Huangshan University,Huangshan 245041;2Department of Material and Chemical Engineering,Guilin University of Technology,Guilin 541004,China);Recent Progress in Chemiluminescence Detection of Amino Acids[J];Amino Acids & Biotic Resources;2007-04
2 WANG Jing-wu *1,PENG Zhi-bing1 and YANG Jia2(1.Department of Chemistry,Nanchang University,Nanchang 330047;2.Tecom Science and Technology Corp.Ltd.Corporration,Nanchang 330029);Determination of chlophenamine maleate in vitamin C yinqiao tablets with capillary electrophoresis-electrochemiluminescence[J];Chinese Journal of Analysis Laboratory;2007-04
3 PENG Zhi-bing,WU Zheng-ping (Medical Aesthetics Research Institute of Yichun College,Yichun 336000,China);Determination of dextromethorphan hydrobromide in oral solution by capillary electrophoresis with electrochemiluminescence detection[J];Chemical Reagents;2008-12
4 PENG Zhi-bing~1,YANG Jia~2,WANG Jing-wu~1(1.Department of Chemistry,Nanchang University,Nanchang 330031,China;2.Tecom Science and Technology Corp.Ltd.Corporration,Nanchang 330029,China);Determination of Benproperine Phosphate in Oral Solution with Capillary Electrophoresis-Eelectrochemiluminescence[J];Journal of Nanchang University(Natural Science);2006-06
5 SUN Bei,SU Xiu-rong,LIN Ye,ZHANG Wei(School of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Determination of parietal collagen in Sipunculid by high performance liquid chromatography[J];Chinese Journal of Biochemical Pharmaceutics;2007-06
6 WU Yuan-yuan~1 LI Li-qing~2 1.Department of Biochemistry,Xingtai University,Xingtai,054001; 2.Department of Chemistry,Taishan University,Tai'an,271021,China;Development of the Research of Chemiluminescence Analysis in Pharmaceuticals[J];Journal of Taishan University;2008-03
7 PENG Zhi-bing1,YANG Jia2,ZHANG Xiao-jun1,YANG Zhi-ming1,WANG Jing-wu1(1.Department of Chemistry,Nanchang University,Nanchang 330031,China;2.Tecom Science and Technology Corp.Ltd.Nanchang 330029,China);Determination of Decloxizine Hydrochloride and Clorprenaline Hydrochloride with Capillary Electrophoresis-Electrochemiluminescence[J];Journal of Instrumental Analysis;2008-01
8 CHENG Mei-rong,WANG Yuan-chao,XIAO Liang(Key Laboratory of Organosilicon Chemistry and Material Technology of Ministry of Education,College of Material Chemistry and Chemical Engineering,Hangzhou Normal University,Hangzhou 310036,China);Determination of Paraquat Residue in Tea by Capillary Electrophoresis with Indirect Electrochemiluminescence Detection[J];Journal of Instrumental Analysis;2009-12
9 LIU Fang, MU Chang-dao* (College of Chemical Engineering, Chengdu 610065, China);Study on Analysis of Hydroxyproline with the Dioxogen Method[J];West Leather;2008-Z1
10 Liu Fang1,Li De-fu1,Lin Wei2,Mu Chang-dao1(1.School of Chemical Engineering,Sichuan University,Chengdu 610065,China;2.The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,Sichuan University,Chengdu 610065,China);Determination Methods and Application of Hydroxyproline[J];China Leather;2007-15
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Chen Xi, Wang Xiaoru, Huang Benli (Department of Chemistry, The research Laboratory of State Education Commission of Analytical Science for Material and Life Chemistry, Xiamen University, Xiamen 361005);The Development of Electrochemiluminescence[J];Chinese Journal of Analytieal Chemistry;1998-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Chang zhi, HAN Mei, DING Bao jun, ZHAO Guo liang ( School of Chem. Eng., Dalian Univ. of Technol., Dalian 116012, China );Electrochemiluminescence of electrodes coated with chitosan encapsulated Ru(bpy)_3~(2+)/silica gel membrane[J];Journal of Dalian University of Technology;2000-05
2 YANG Ji-dong;Application of Chemiluminescence Analysis in the Environmental Analysis and the Pharmaceutical Analysis[J];Journal of Fuling Teachers College;2001-04
3 Wang Peng, Yuan Yi, Zhu Cuoyi, Zhang Milin (Laboratory of Eleanmdytical Chemistry, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022) (Department of Chemistry Engineering, Harbin Engineering Unviersity, Harbin 150001);Progress in Electrochemilulnmescent Analysis[J];Chinese Journal of Analytical Chemistry;1999-10
4 ZHU Lian de, ZHU Guo yi *(State Key Laboratory of Electroanalytical Chemistry, Changchun Institute ofApplied Chemistry, Chinese Academy of Sciences, Changchun 130022);Recent Developments in Electrochemiluminescence Sensors[J];Journal of Analytical Science;2003-03
5 LI Ying xiu, ZHU Lian de and ZHU Guo yi (Laboratory of Electroanaltical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022);An optical fiber biosenor based on electrochemiluminescence for glucose analysis[J];Analytical Laboratory;2001-05
6 QI Ying-ying, WU Ying, DI Jun-wei, TU Yi-feng*College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China;The Determination of Glutathione by ElectrochemiluminescenceQuenching Method[J];Spectroscopy and Spectral Analysis;2005-02
7 CHU Hai-hong, QI Ying-ying, XU Yang, HUANG Bing-qiang, TU Yi-feng~* College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China;Determination of I~- by the ECL of Luminol in Neutral Medium[J];Spectroscopy and Spectral Analysis;2005-05
8 LUO Lirong~1 ZHANG Zhujun~2 1.Exploration and Development Research Institute,Changqing Oilfield Company of Petrol China,Xi'an 710021;2.School of Chemistry and Materials Science,Shaanxi Normal University,Xi'an 710062;On Line Galvanic Cell Generated Chemiluminescence[J];Journal of Ningxia Teachers University;2008-03
9 PENG Zhi-bing~1,YANG Jia~2,WANG Jing-wu~1(1.Department of Chemistry,Nanchang University,Nanchang 330031,China;2.Tecom Science and Technology Corp.Ltd.Corporration,Nanchang 330029,China);Determination of Benproperine Phosphate in Oral Solution with Capillary Electrophoresis-Eelectrochemiluminescence[J];Journal of Nanchang University(Natural Science);2006-06
10 ZHANG Chengxiao QI Honglan (School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062);Progress in Electrogenerated Chemiluminescence Analysis[J];World Sci-tech R & D;2004-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Guan Jing Ye Ping Wu Jimin (Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 300161);The determination of hydroxyproline in collagen sponge[J];AMINO ACIDS & BIOTIC RESOURCES;2000-01
2 Lu ping;Zhang Linsheng;Cao Rang(Central Laboratory,Northwestern Agricul tural University,Yanglin,shanxi 712100,PRC);Studies on the Determination Method of Hydroxyproline in Animal Adipose Tissue[J];;1994-02
3 Liu Zhiguang and Ma Haiyan(Department of Pathology, The Fourth Military Medical University);Research and Application of a Fast Method to Determinate Collagen Specific Amno Acids[J];;1994-03
4 CAO Chengbo 1),LV Ronghui 1),ZHANG Chunlian 2),ZOU Yuping 1),SONG Guodong 3),ZHANG Changqiao 1)1)Chemistry and Chemical Engineering School,Shandong University,Jinan 250100,China 2)Shandong University Hospital,Jinan 250061,China 3)Jinan Central Hospital,Jinan 250013,China;Properties and structure characterization of new type collagen scaffold materials[J];Journal of University of Science and Technology Beijing;2007-02
5 XIE Li 1, WEN Ai-Dong 1, LUO Xiao-Xing 2, WU Yin 1, YANG Zhi-Fu 1, CHEN Pin-Jun 1, ZHAO Lei 1, LI Wei 1 1Department of Pharmacy, Xijing Hospital, 2Department of Pharmacology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Pharmacokinetics of pazufloxacin mesylate injections in healthy volunteers[J];Journal of The Fourth Military Medical University;2005-09
6 ZHOU Xue zhang 1,DENG Xu ming 1,YAN Ji ye 1 ,CHENG Yuan guo 2 (1.Faculty of Veterinary Medicine,University of Agriculture and Animal Sciences, Changchun 130062,China;2.Academy of Military Medical Sciences,China);Determination of Clindamycin by RP-HPLC[J];Progress In Veterinary Medicine;2002-03
7 Jin Jian Guan Ling Wang Bocheng Luo Shineng (State Key Laboratory of Nuclear Medicine, Wuxi 214063);EVALUATION OF OPTIMAL PARAMETERS FOR ACRIDINIUM ESTER LABELED CHEMILUMINESCENE IMMUNOASSAY[J];CHINESE JOURNAL OF LUMINESCENCE;1997-03
8 SHEN Xiao-dong, SONG Guan-bin, YAN Run-bin, YANG Yang (Key Laboratory for Biomechanics and Tissue Engineering Under the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China);Research Progress of Matrine and Oxymatirne in the Anti-tumor Mechanism[J];Journal of Chongqing University(Natural Science Edition);2005-06
9 XIE Cheng gen(Department of Chemistry,Wanxi College,Lu′an 237012);Flow Injection Analysis of Tyrosine withEnhanced Chemiluminescent Detection[J];Journal of Huaibei Industry Teachers College (Natural Sciences Edition);2002-02
10 An Jingru Lin Jinming Chen Xi (Department of Chemistry,Fuzhou University,Fuzhou 350002);Eletrochemiluminescence and Its Applications in Analysis[J];Chinese Journal of Analytical Chemistry;1991-11
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YAN Mei,HAN Yan-yan,XU Yuan-yuan,XIA Yang,CAI Yan-yan,ZHANG Li-na,WEI Qin(School of Chemistry & Chemical Engineering,University of Jinan,Jinan 250022,China);The Application of Capillary Electrophoresis to the Pharmaceutical Separation and Analysis[J];Analysis and Testing Technology and Instruments;2008-02
2 ZENG Nuan-xi1,WANG Hong-jian1,ZHOU Xing-qi2,FENG Zhi-qiang1 (1.Guangdong Food Quality Supervision and Inspection Station,Guangzhou 510308,China) (2.Guangdong Provincial Public Laboratory of Food Industry,Guangzhou 510308,China);Determination of Hydroxyproline in Dairy Products by Amino Acid Autoanalyzer[J];Modern Food Science and Technology;2008-07
3 ZHOU Yan-gang, JIA Jian-ping, LU Jian-zhang, CHENG Min, YE Xiao-di, ZHENG Gao-li(Institute of Health Food, Zhejiang Academy of Medical Sciences, Hangzhou 310012,China);Determination of Hydroxyproline in the Collagen of Fish Skin by Hydrolysis under Vacuum[J];Modern Food Science and Technology;2009-11
4 YU Xi-juan,MOU Chuan-qin (College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Shandong Qingdao 266042,China);Applications of Chemiluminescence Detection for Capillary Electrophoresis in Drug Analysis[J];Guangzhou Chemical Industry;2009-08
5 SHAO Liang-liang, SU Xiu-rong, LI Yan-yan, XU Cai-yun, XU Jia-jie, HOU Fu-jing(Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211);Study on changes of amino acid content of rice during storage[J];Food Science and Technology;2009-07
6 LAN Su-mei1,LI Li-jun2,CAI Zhuo1,CHENG Hao2,CHEN Chang-dong1 (1.College of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China;2.Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou 545006,China);Determination of Clindamycin by Using Enhanced Ru(bpy)_3~(2+) Electrochemiluminescence[J];Journal of Instrumental Analysis;2009-04
7 LUO Feng-lian,XIA Yan-bin,OUYANG Jian-xun,WANG Yan,XIA Xiao-feng (College of Food Science and Technology,Hunan Agricultural University,Changsha 410128);Determination of hydroxyproline in chicken by spectrophotometer[J];Food Science and Technology;2010-01
8 CHENG Xian-long~1 XIAO Xin-yue~1 ZOU Qin-wen~2 LI Guang-hua~3 TIAN Shou-sheng~4 ( 1. National Institute for the Control of Phamaceutical and Biological Products, Beijing 100050, China; 2. Beijing University of Chinese Medicine,Beijing 100029 ,China; 3. Liaocheng Institute for Drug Control,Liaocheng 252000, China; 4. Shandong Dong E E Jiao CO. , LTD. , Liaocheng 252201, China);Pre-column derivatization HPLC simultaneous determination of 4 main amino acids in donkey-hide glue[J];Chinese Journal of Pharmaceutical Analysis;2008-12
9 BU Sheng-gao(Institute for Drug Control of Yongzhou,Lingling Hunan,425100);HPLC determination of benproperine phosphate in benproperine phosphate capsules[J];Central South Pharmacy;2009-02
10 XU Cai-yun,SU Xiu-rong,LI Yan-yan,HOU Fu-jing,SHAO Liang-liang,XU Jia-jie (Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);THE NUTRIENTS CONSTITUENTS OF ACAUDINA LEUCOPROCTA AND ITS HYPOLIPIDEMIC EFFECT[J];Acta Nutrimenta Sinica;2009-04
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 An Jingru, Chen Xi (Department of Chemistry, Fuzhou University, Fuzhou);Electrochemiluminescence of ABEI in Aqueous Alkaline Solution[J];Chemical Research In Chinese Universities;1989-11
2 An Jing-ru, Lin Jin-ming, Xie Zeng-hong (Department of Chemistry, Fuzliau University, Fuzhou, 350002);Determination of Rare Earth Element Yttrium by Electrogenerated Chemiluminescence of ADPD[J];Chemical Research In Chinese Universities;1991-05
3 An, Jing-Ru~* (Department of Chemistry, Fushou University, Fuehou, 350009) Lin, Jian-Guo (Wuhan Institute of Control and Examine of Quality of the Products, Wuhan, 430015);Investigation of Electrochemiluminescence Immunoassay for Determination of HCG[J];Acta Chimica Sinica;1992-06
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