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《Chinese Journal of Analytical Chemistry》 2006-03
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Protein Phosphatase Inhibition Assay for Detection of Diarrhetic Shellfish Poison in Oyster

Li Aifeng~ 1,2 , Yu Rencheng~ *1 , Li Jun~1, Tang Xianghai~ 1,2 , Wang Yunfeng~1, Yan Tian~1~1, Zhou Mingjiang~1 ~1(Key Laboratory of Marine Ecology and Environment Sciences, Institute of Oceanology,Chinese Academy of Sciences, Qingdao 266071) ~2(Graduate School, Chinese Academy of Sciences, Beijing 100039)  
A method based on protein phosphatase enzyme activity inhibition for the detection of diarrhetic shellfish poison (DSP) was used to analyze the DSP toxicity in three oyster samples. Based on the standard dose-effect curve developed with a series of okadaic acid (OA) standard solutions, the DSP toxicity of the three oyster samples collected were screened, and the results showed that there were no OA and dinophysis toxins (DTXs) in the samples without hydrolization. However, the OA toxicity could be detected in two of the hydrolyzed samples, and the OA toxicity of the two samples were 1.81 and 1.21 μg OA eq./kg oyster, respectively .
【Fund】: 国家十五科技攻关计划(No.2001BA804A20);; 973国家重点基础研究课题(No.2001CB409704);; 中科院海洋研究所创新项目(No.200223107)资助
【CateGory Index】: X174
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