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《Chinese Journal of Analytical Chemistry》 2006-05
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The Distribution of Podophyllotoxin in the Different Parts of Dysosma Versipellis (Hance) M. Cheng by Capillary Zone Electrophoresis

Deng Shuduan, Zhao Xingling, Wang Wei , Huang Qiling, Ding Zhongtao, Cao Qiue 1 (Department of Chemistry, Center for Advanced Studies of Medicinal and Organic Chemistry,Yunnan University, Kunming 65009)  
A simple and sensitive capillary zone electrophoresis (CZE) method for the determination of podophyllotoxin (PPT) has been developed, and the distribution of podophyllotoxin in the different part of Dysosma versipellis (Hance) M. Cheng was studied by the proposed method. The results indicated that podophyllotoxin could be separated availably from the other constituents in the different part of Dysosma versipellis (Hance) M. Cheng in an uncoated fused-silica capillary (57 cm×75 μm i.d. with the effective length of 50 cm) using 25 mmol/L borate with pH 10.8 as running buffer. The linear range for the determination of podophyllotoxin was 1.5-171.0 mg/L with the relative standard deviations (n=8) for the relative migration time and peak area of 1.5% and 0.8%, respectively. The results obtained from the determination of podophyllotoxin in the ethanolic extracts of the roots, stems, leaves and fibrils of Dysosma versipellis (Hance) M. Cheng pointed out that the distribution of podophyllotoxin in the different part of Dysosma versipellis (Hance) M. Cheng was not equal . Podophyllotoxin in the roots was highest, and in the leaves was lowest,
【Fund】: 国家自然科学基金(No.30260014 20465003);; 云南省人才培养基金(No.2004PY01~4);; 云南省高等学校教学、科研带头人资助项目
【CateGory Index】: O658.9
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