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《Chinese Journal of Analytical Chemistry》 2006-09
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Linear Sweep Polarographic Determination of Hyaluronic Acid with Safranine T as Electrochemical Probe

Sun Wei~*,Gao Ruifang,Ding Yaqin,Jiao Kui (College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266042  
In pH 1.5 Britton-Robinson buffer solution,safranine T(ST) has a sensitive second order derivative linear sweep polarographic reductive wave at-0.35 V(vs.SCE) on drop mercury working electrode. After the addition of hyaluronic acid(HA) to the ST solution,a supramolecular complex was formed based on the electrostatic attraction of positively charged ST with negatively charged HA,which resulted in the decrease of the free concentration of ST in the solution,and the decrease of peak current.The condition of the binding reaction and the electrochemical detection were optimized.Under the optimum conditions,the i~″_p of ST decreased proportionally to the HA concentration from 3.0 to 100.0 mg/L with the linear regression equation as Δi~″_p(nA/s~(-2))=27.23+15.18C(mg/L)(n=14,r=0.996).The method bas been applied to the determination of HA synthetic samples with satisfactory results and the binding number was calculated as 870 by molar ratio method.
【Fund】: 国家自然科学基金(No.20405008 20375020);; 青岛市自然科学基金(No.04-2-JZ-114);; 青岛科技大学博士基金(No.0022125)资助项目
【CateGory Index】: O657.14
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