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《Chinese Journal of Analytical Chemistry》 2009-05
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Glucose Oxidase Biosensor Based on Carbon Nanotubes and Chitosan Through Electrostatic Adsorption

CHEN Wen-Jing,QU Jian-Ying(College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004)  
A novel glucose biosensor was fabricated by means of self-assembled technique to immobilize glucose oxidase(GOD).Neutral Red was electorpolymerized on glassy carbon electrode(GCE) modified with Nafion to form a positively charged surface,then multi-walled carbon nanotubes(MWNTs) and chitosan(Cs) was linked by electrostatic adsorption step by step,finally GOD was electrostaticly absorbed in Cs film to prepare a glucose biosensor.The experiments showed a linear range to concentration of glucose was 5.0×10-6-2.0×10-3 mol/L with a detection limit of 1.0 ×10-6 mol/L in PBS(pH 7.0) at 30 ℃,and response time was less than 10 s.The biosensor retained 84.3% of its original peak current after thirty days.Moreover,the biosensor exhibited good reproducibility,high selectivity and anti-interference ability to ascorbic acid,etc.
【CateGory Index】: TP212.2
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