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《Chinese Journal of Analytical Chemistry》 2011-03
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Determination of Total Main Ginsenosides Contents in American Ginseng and Chinese Ginseng Using Near Infrared Spectroscopy

HUANG Ya-Wei1,WANG Jia-Hua1,Jacqueline J.Shan2,LEI Ling2,HAN Dong-Hai11(College of Food Science & Nutritional Engineering,China Agricultural University,Beijing 100083)2(Afexa Life Sciences Inc.Edmonton,T6N 1G1,Canada)  
Potential of NIR spectroscopy to determine total main ginsenosides contents in Chinese ginseng and American ginseng was studied.Diffuse reflective spectra of all samples were collected.Then the raw spectra were preprocessed with orthogonal signal correction(OSC) and routine methods respectively.After that,the corresponding partial least-squares(PLS) regression models were developed.Comparing with the optimal routine spectral preprocessing method,OSC can eliminate the sample species differences perfectly and greatly enhance the correlation between the spectra and the analyte of interest.Meanwhile,OSC decreased the PLS factors for modeling,which made the model more robust.The correlation coefficient of prediction(Rp),root mean standard error of prediction(RMSEP) and ratio of deviation to performance(RPD) of the OSC-PLS model finally developed were 0.97,0.519 and 4.07 respectively.The result indicated that NIR spectroscopy could be used in practice to fast determine the total main ginsenosides contents in Chinese ginseng and American ginseng and fulfill the screen of raw materials on site.
【Fund】: 中加合作研究项目(No.200904810610008)资助
【CateGory Index】: S567.5;O657.33
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