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《Chinese Journal of Analytical Chemistry》 2012-08
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Analyses of Hemolytic Toxin from Ichthyotoxic Phytoplankton Chattonella marina(Hong Kong Strain) by High Performance Liquid Chromatography

WU Ni,JIANG Tian-Jiu*,JIANG Tao(Research Center for Harmful Algae and Aquatic Environment,Jinan University,Guangzhou 510632,China)  
Chattonella marina is a typical fish-killing HAB(Harmful Algae Bloom) species,which could produce hemolytic toxin and result in mass mortality of fishes.In order to understand the transmission and transformation of hemolytic toxin in food chain,high performance liquid chromatography(HPLC) with gradient elution has been used to separate hemolytic toxin extracted from C.marina.Different solvent systems,detection wavelength,isocratic elution and gradient elution for HPLC were compared.The separation is carried out on a C8 column(4.6 mm×150 mm,5 μm) with acetonitrile/water as mobile phase,gradient elution(0-10 min 60%-80% acetonitrile,10-40 min 80%-90% acetonitrile,40-50 min 90% acetonitrile) with a flow rate of 0.8 mL/min.The detection wavelength is 205 nm and 448 nm,and the column temperature is 25 ℃.Hemolysis bioassay shows that there are at least five components in C.marina(Hong Kong stain).One component had the maximum ultraviolet(UV) absorption at 448 nm,another that was not separated completely had the maxima at 440 nm and 446 nm,and the others all had the maximal peak at 205 nm.
【Fund】: 国家自然科学基金项目(No.41106090);; 国家863项目(No.2008AA10Z427);; 国家重点基础研究发展规划项目(No.2010CB428702);; NSFC-广东联合基金项目(No.U0733006)资助
【CateGory Index】: X835;O657.72
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