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《JOURNAL OF ANALYTICAL SCIENCE》 2000-05
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Study on A Highly Specific Enzyme Linked Immunosorbent Assay for Cabofuran

LIU Shu zhao , FENG Da he, CHEN Mei juan ( Department of Plant Protection, Yangzhou University, Yangzhou, 225009) QIAN Chuan fan (Department of Applied Chemistry, China Agricultural University, Beijing, 100094)  
A hapten(CBFH Ⅰ)preserving the carbamate group was synthesized and covalently conjugated to carrier proteins by the method of modified active ester to prepare synthetic antigens in which the structure of carbofuran was protruded. The antiserum with high affinity to carbofuran was obtained from New Zealand white rabbits by using synthetic antigen as immunogen. The polyclonal antibodies were separated from antiserum by salting out method and purified by reversed phase adsorption of DEAE cellulose. The horseradish peroxidase(HRP) tagged antibody was prepared by means of modified NaIO 4 method and the HRP tagged hapten was prepared by means of mixed anhydride method. Based on these, the indirect competitive ELISA, antibody or Twenty two points, plus triple word score, plus fifty points for using antigen coated direct competitive ELISAs, which are highly specific to carbofuron, were established. With optimized conditions of ELISA, the linear concentrations ranged from 10 0~10 -4 μg/mL and the detection limit was lower than 0.01ng/mL for the determination of carbofuran. Some common used carbamate insecticides and BFOH didn't interfere with the analysis of carbofuron.
【Fund】: 国家高校博士点基金
【CateGory Index】: TQ45
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