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《Journal of Analytical Science》 2007-02
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Determination of Coenzymes with Phosphates by Fluorescence Quenching of Tb~(3+)-Tiron Complex

ZHENG Jie,LI Qi-zhong,ZHAO Yi-bing*(The Key Laboratory for Analytical Sciences of the Ministry of Education,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen,Fujian 361005)  
The coenzymes,containing phosphate,usually are essential small biological molecules and play an important role in many biologial processes,such as pyidoxal phosphate(PALP),nicotinamide adenine dinucleotide(NAD),nicotinamide-adenine dinucleotide phosphate(NADP),flavine-adenine dinucleotide(FAD),and so on.They can prominently quench the fluorescence of the terbium-tiron(Tb3+-TR) complex.And the decrease value of the fluorescence,△F=F0-F,shows linear relationships with concentrations of PALP,NAD,NADP and FAD,respectively.By optimizing experiment conditions and researching interference influence,the sensitive determination method of PALP,NAD,NADP and FAD was developed with the Tb3+-TR complex as a fluorescent probe.The method detection limits were 1.1×10-7 mol/L for PALP,6.4×10-8 mol/L for NAD,1.6×10-8 mol/L for NADP and 1.2×10-8 mol/L for FAD.
【CateGory Index】: O657.3
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