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Immobilization of Hemoglobin on Magnetic Microspheres for Preparation of Hydrogen Peroxide Biosensor

ZOU Xiao-zhi1,2,ZHANG Hai-li*1,2,WANG Fang1,2,HAN De-yan1,2 (1.Hubei Key Laboratory of Bioanalytical Technique,Department of Chemistry,Hubei Normal University,Huangshi,Hubei 435002;2.Department of Chemistry,Central China Normal University,Wuhan 430079)  
A third-generation hydrogen peroxide biosensor was designed by immobilizing Hb on a glassy carbon electrode modified with natural polymer magnetic microspheres.It was found that the electron transfer between Hb and the underlying electrode was greatly enhanced in the advantageous microenvironment with the natural polymer magnetic microspheres.The fabrication procedures and electrochemical behaviors of protein were characterized by electrochemical impedance spectroscopy and cyclic voltammetry.The modified electrode exhibited excellent electrocatalytic activity and rapid response for H2O2.The electrocatalytic response showed a linear dependence on H2O2 concentration ranging from 5.2×10-5 mol/L to 2.3×10-3 mol/L with a detection limit of 8.7×10-6 mol/L.In addition,the biosensor had good repeatability and long-term stability.
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