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《Journal of Analytical Science》 2008-01
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Determination of Phenol in Environmental Sample by Using the Mimetic Enzyme Catalytic Reaction

HUANG Ying-ping*,FANG Yan-fen,XU Wen-nian,DAI Fang-xi,SONG Qiu-ju,ZHAO Cai-xia,ZHANG Yi(Research Center for Eco-Environmental Sciences,China ThreeGorges University,Yingchang,Hubei 443002)  
Base on the chromogenic reaction of enzyme catalytic reaction oxidase hydrogen peroxide with Hemin as a mimetic peroxidase,4-aminoantipyrine and phenol as hydrogen donator substrate,a method for determination phenol compounds was established.The apparent molar absorption for determination of phenol was found to be 1.73×105 L·mol-1·cm-1 at pH 11.2.The good lineraty was obtained in range of phenol concentration of 0~7.38×10-6 mol/L.Determination of concentration of phenol in the wastewater was carried out with satisfactory results.
【Fund】: 国家自然科学基金(No.50639070 20373074);; 湖北省青年杰出人才基金(No.2005ABB030);; 湖北省教育厅自然科学重大基金研究项目(No.2004Z001)
【CateGory Index】: X832
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