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《Journal of Analytical Science》 2011-01
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PAMAM Dendrimer Modified Silica as Carriers for Trypsin Immobilization

DING Fang-yu,SU Ping,ZHANG Chun-ting,E Hong-jun,YANG Yi(College of Science,Beijing University of Chemical Technology,Beijing 100029)  
PAMAM dendrimer modified silica was prepared for trypsin immobilization.The generation of PAMAM and the immobilization conditions were investigated.The results showed that the 3.0 G PAMAM dendrimer modified silica would provide high immobilization yields and enzymatic activity and it was employed for trypsin immobilization.The optimum temperature and pH of the immobilized enzyme were 60℃ and 9.0,respectively.The Km value for casein was 7.76 mg/mL.Compared with free enzyme,the immobilized enzyme has better thermo-stability,and it also features good storage stability and reusability.
【Fund】: 国家自然科学基金(No.90713013)
【CateGory Index】: Q814.2
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