Study on Screening of Anticancer Drugs by Lymphocyte Nuclear FDNA Acridine Orange System Fluorescence Inhibition
Chen Bei, Tang Hongwu, Chen Guanquan, Ye Yong, Zeng Yune (Department of Chemistry, Department of Analysis and Measurement Science, Wuhan University, Wuhan, 430072)
Using human lymphocyte as standard cell and acridine orange (AO) to trace the interaction of drug and cellular DNA, a novel method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis is developed. The results for five Cell Cycle Nonspecific Agents (CCNSA) are in accordance with the mechanism of the drugs binding to cellular DNA, this indicates that this method is suitable for screening anticancer drugs of this kind. The study of four metal complexes of Schiff bases derived from chlorobenzaldehyde and L alanine shows that: all of the four compounds 3 CACo 、3 CAZn、2 CAK and 4 CAK can bind to nuclear DNA to an extent, but they are not sensitive for G 0 phase lymphocyte.