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《Analytical Laboratory》 2003-01
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Determination of hydrogen peroxide using hemoglobin as mimetic peroxidase

HUANG Ying-ping, LIU Feng, CHEN Bai-ling, LUO Guang-fu, YAN Ke-mei, LI Yang (Department of Chemistry, Three Gorges University, Yichang, 443003),Fenxi Shiyanshi,2003,22(1):12~15  
The catalytic activity of hemoglobin to oxidation of Recessive Brilliant Green (RBG) by peroxide hydrogen was studied. Also, catalytic characteristic and reaction condition of Hb-RBG-H 2O 2 system were discussed. At pH 5.73, products of this system exhibited maximum absorption at 640 nm. The apparent molar absorption for determination of hydrogen peroxide was found to be 7.15×103 L·mol -1·cm -1 with a detection limit of 2.8×10 -6 mol/L. The method was used for determination of trace H 2O 2 with good results.
【Fund】: 湖北省教育厅自然科学基金资助项目 (2 0 0 1B140 0 5 )
【CateGory Index】: O657.32
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