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《Analytical Laboratory》 2005-04
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Determination of Pyruvate by Ultraviolet Spectrophotometry

LOU Liang-wang~ and GAO Nian-fa (Department of Bio-engineering, Tianjin University of Science and Technology, Tianjin 300222), Fenxi Shiyanshi, 2005, 24(4): 11~13  
A method for the determination of pyruvate was studied by ultraviolet spectrophotometry in this paper. At pH 11.0 and 320 nm, pyruvate achieved higher absorptivity than other organic acids and coexistent components. Beer′s law was obeyed for pyruvate in the concentration range from 0.2 to 2.8 g/L. This method is simple and has high selectivity for the determination of pyruvate in the fermentation broth and its product.
【CateGory Index】: O623.5
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