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《Analytical Laboratory》 2005-04
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Separation and determination of active components of marchantia polymorpha flavonoids extract by RP-HPLC

XIAO Jian-bo~*, ZHU Hua, ZHONG Shi-an, ZHOU Chun-shan (Central South University, Changsha 410083), ZHU Hua and HUANG Hai-bin (GuangXi Chinese Medical University, Nanning 530001), Fenxi Shiyanshi, 2005, 24(4): 17~19  
A method for the separation and determination of Marchantia Polymorpha flavonoid extract by reversed-phase high performance liquid chromatography (RP-HPLC) was proposed. The components can be separated through a C_(18) analytical column with acetic acid-methanol-acetonitrile-phosphoric acid-water as mobile phase in 30 min. Meletin, cyanidenon and versulin were quantitated by calibration curve method at detection wavelength of 350 nm. The result shows that the average recovery is 96.79%~101.13%, RSD is 0.66%~1.5%.
【Fund】: 桂科攻 (0 2 35 0 2 2 - 1B) ;; 桂科回 (0 0 0 90 0 4 )
【CateGory Index】: TQ461
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