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《Chinese Journal of Analysis Laboratory》 2007-07
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Study on HPLC method for determination of five kinds of organic acids in flos lonicerae

ZHANG Yu-ping and HUANG Ke-long(Centural South University,Changsha 410083)  
An HPLC method with gradient elution mode is developed for the simultaneous separation and determination of chlorogenic acid,caffeic acid,3,5-O-dicaffeoylquinic acid,3,4-O-dicaffeoylquinic acid and 4,5-O-dica-ffeoylquinic acid in flos lonicerae.These five organic acids can be extracted from the flos lonicerae samples with 80% of methanol.Experiments were performed on waters symmetry○R RP18 column(3.9×250 mm,5 μm),with methanol and acetic acid solution(0.1%) as mobile phase using gradient elution at a flow-rate of 0.8 mL/min and detected with a photodiode array detector.The recoveries of five organic acids are 97%~101%,and the relative standard deviations are 0.3%~2.4%.This method can be applied to the determination of five organic acids in flos lonicerae with satisfactory results.
【CateGory Index】: R284
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2 NIU Peng-fei, QIU Nong-xue (College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062);Development of a RP-HPLC Method for Simultaneous Determination of Lactic Acid,Fumaric Acid, 5-HMF and Patulin in Apple Juice[J];Scientia Agricultura Sinica;2008-04
3 SONG Hong-jie1,XIE Jiang2,GAO Hong-bin1,WANG Zhi-jun1,WU Ming-sheng1(1.Department of Pharmacy,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Postgraduate Team in School of Pharmacy,Fudan University,Shanghai 200032,China);Determination of content of total organic acid in Ganmao'an granules and its application[J];Pharmaceutical Care and Research;2009-04
Chinese Journal Full-text Database 1 Hits
1 Wang Tianzhi,Li Yongmei and Wang Zhixiao (School of Pharmacy,West China University of Medical Sciences,Chengdu 610041);Determination of Three Kinds of Organic Acid in Honeysuckle Flower by RP-HPLC[J];CHINESE JOURNAL OF PHARMACEUTICAL ANALYSIS;2000-05
Chinese Journal Full-text Database 10 Hits
1 WANG Ju-tao,LIU Jin-song,XIE Dong-mei (Anhui College of Traditional Chinese Medicine,Hefei 230001);Determination of chlorogenic acid in xiaoerkechuanling gel by HPLC[J];Anhui Medical and Pharmaceutical Journal;2007-02
2 Wu Shu-jun (The First People's Hospital of Xinxiang City,Xinxiang 453000,China);Improvement of the Quality Standards of Qingrejiedu Oral Solution[J];Chinese Journal of Medicinal Guide;2008-08
3 PENG Shu-qin, XIE Shuang-xi Forest College of Guizhou University, Guiyang 550025, China;Preliminary Analysis on Biological Characters of Honeysuckle (Lonicera japonica)[J];Guizhou Agricultural Sciences;2003-05
4 XIE Bi-xiu SUN Zhi-da (Department of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China);Progress of Researches on the Main Organic Acids in Honeysuckle[J];Modern Food Science and Technology;2007-09
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6 CHENG Tie-feng1,ZHU Xin-ke2,XU Qi-tai1 (1.Institute of Chinese Medical,Pharmacy Collage of Henan university,Henan Kaifeng 475001,China;2.Kaifeng Heda Pharmaceutical Factory,Henan Kaifeng 475001,China);Determination of caffeic acid in leaves and roots of Herba taraxacum mongolicum by RP-HPLC[J];Journal of Henan University(Medical Science);2006-04
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9 HUANG Xi-ru SHEN Peng Hebei Medical University;Research Advancement on Fingerprints Chromatogram and Quality Criteria of Flos Lonicerae Japonicae and its Preparations[J];Shanghai Journal of Traditional Chinese Medicine;2006-10
10 ZHANG Jun1, ZHUANG Gui-dong, HAN Rong-wei, QIU Hong-wei, XIAO Jun-xia, CHI Yu-sen (1.Department of Food Science ,Qingdao Agricultural University , Qingdao 266109, Shandong, China);STUDY ON THE METHOD OF TLC-UV DETERMINE THE CONTENT OF CHLOROGENIC ACID FROM FLOS LONICERAE[J];Food Research and Development;2008-03
China Proceedings of conference Full-text Database 1 Hits
1 HU Run-huai WAN Yan (Zhejiang Forestry University,Zhejiang Lin' an 311300) (Pharmacy college,Henan University of Traditional Chinese Medicine,Zhengzhou 450008);The Determination of the content of chlorogenic acid in Folium Lonicerae in different regions and periods[A];[C];2007
Chinese Journal Full-text Database 10 Hits
1 WU Zhen-zhen1,2,3,LIN Zhi-fen1,4,5,YIN Da-qiang4,YIN Ke-dong1(1 Key Laboratory of Tropical Marine Environmental Dynamics,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;2 Haikou Marine Environmental Monitoring Central Station,State Oceanic Administration,P.R.China,Haikou 570311,China;3 Graduate University of Chinese Academy of Sciences,Beijing 100039,China;4 Key Laboratory of Yangtze River Water Environment,Ministry of Education,College of Environmental Science and Engineering,Tongji University,Shanghai,200092,China;5 State Key Laboratory of Marine Environmental Science,College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,Fujian,China);Development of solid phase micro-extraction for the environment monitoring[J];Journal of Safety and Environment;2008-02
2 DING Ling, TU Youying, CHEN Xiaomin (Department of tea science, Zhejiang University, Hangzhou 310029, China);Simultaneous Determination of Various Organic Acids in Compressed Tea by High Performance Liquid Chromatography[J];Journal of Tea;2005-04
3 Guo Jia-xin;Extraction of the Cresol from Waste Water by Castor Fatty Acid[J];China Surfactant Detergent & Cosmetics;1991-04
4 GUAN Jie,WANG Zong-hua,SUN Xi-quan,ZHANG Fei-fei,XIA Yan-zhi(Laboratory of Fiber Materials and Modern Textile,the Growing Base for State Key Laboratory,Qingdao University,Qingdao 266071,Shandong,China);Enhancing Spectral Probe Based on the Synergic Effect of Carbon Nanotubes and PVA for the Sensitive Determination of Heparin[J];Journal of Materials Engineering;2008-10
5 Han Ying Zhang Jing Ji Xin Department of Gastroenterology,Beijing Army General Hospital,Beijing 100700,China;N-Acetyicysteine Alleviate the DSS Induced Experimental Colitis in Mice through Replenishment of Glutathione Level[J];Chinese Journal of Medicinal Guide;2008-01
6 ZHAO Ping~1,DONG Lei~1,LUO Jin-Yan~1,MA Hui~1,GUAN Hai-Tao~2,SHANG He-Ling~3~1Department of Gastroenterology,~2Department of Oncosurgery,Second Hospital,Xi'an Jiaotong University,Xi'an 710004,(China,) ~3Tangyu Convalescent Hospital,Lantian 710516,China;Establishment of dextran sulfate sodium-induced ulcerative colitis model in rats[J];Journal of The Fourth Military Medical University;2005-19
7 WANG Xiao-zhou1,LIU Chang-lu1,JIA Xiao-yan2(1.Department of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou 635000,China; 2.School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China);Determination of Dextran Sulfate Sodium with Cetylpyridinium Bromide by Resonance Rayleigh Scattering[J];Sichuan University of Arts and Science Journal;2008-02
8 He Caixia Lu Minggang Cui Hua Zhao Guiwen (Departmetn of Applied Chemistry, University of Science and Technology of China, Hefei 230026);THE DETERMINATION OF CHLOROGENIC ACID BY CHEMILUMINESCENCE ANALYSIS[J];CHINESE JOURNAL OF LUMINESCENCE;1997-01
9 LI Fang, JIANG Zhi-liang (Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004; China);Resonance Scattering and Fluorescence Quenching and Hypochromic Effects of (PtI_6-2RDG)_n Association Nanoparticle[J];Analysis and Testing Technology and Instruments;2002-03
10 Zhang Lan, LIU Yuan-huan, HE Yu, CHEN Guo-nan(Department of Chemistry, Fuzhou University, Fuzhou 350002, China);The Simultaneous Determination of Chlorogenic Acid and Quercetin in Folium Pyrrosiae by Capillary Electrophoresis with Electrochemical Eetection[J];Analysis and Testing Technology and Instruments;2005-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Xu Bing-sheng (Department of Biology, Futan University, Shanghai);A BOTANICAL STUDY OF THE CHINESES DRUG JIN-YIN-HUA[J];Acta Pharmaceutica Sinica;1979-01
2 Xu Guojun;Xu Luoshan;Niu Yanzhen;Xu Bingsheng;Studies on the Identification of the Chinese Drug Jin-Yin-Hua[J];;1979-01
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