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Determination of total glucoside content of paeony in Paeonia lactifloria Pall.by Alkaline hydrolysis-UV spectrophotometry

REN Feng-lian,QU Bai-lu and TAN Xiao-yan(College of Chemistry and Chemical Engineering,Central South University,Changsha 410083)  
A method was proposed for the determination of the content of total glucosides of paeony in Paeonia lactiflora Pall.by UV spectrophotometry.The determination method was established with benzoic acid which was hydrolysis product of total glucosides of paeony as a contrast by UV spectrophotometry at 228 nm.A good linear relationship between concentration of benzoic acid and its absorbance was obtained,and the linear range was between 2.0 and 10.0 μg/mL with correlation coefficient of r=0.9984.The average recovery(n=6) and RSD were 99.1% and 2.4% respectively.This method is simple rapid and reliable.It could be used for the semi-quantitative determination of total glucosides of paeony in Paeonia lactifloria Pall..
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