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《Chinese Journal of Analysis Laboratory》 2011-02
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Determination of the contents of total flavonoids in lonicera japonica and its leaves by three wavelength spectrophotometry

WANG Ke1,WANG Yong1,ZHAO Dong-bao1,3,LI Ming-jing1,LIU Xiu-hua1,2 and XU Ping-hui4(1.Environmental and Analytical Science Institute of College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004;2.Key Lab of Natural Drug and Immune Engineering,Henan Province,Kaifeng 475004;3.Jiyuan Vocational and Technical College,Jiyuan 454650;4.Xinxiang Biocaro biotech co.LTD,Xinxiang 453000)  
A three wavelength spectrophotometry for the determination of the contents of total flavonoids in Lonicera japonica and its leaves was studied by using 5,7-dihydroxy-6,4′-dimethoxyflavone as a reference substance and aluminum trichloride as a colorimetric reagent.The linear range of the method was 1.92~19.20 μg/mL with the correlation coefficient of r=0.9997.The average recoveries were 96.6%,97.1%,and RSDs were 1.4%,2.3%(n=4).By selecting the three wavelengths appropriately,the absorbance of interfering components can be corrected efficiently.The method is feasible for the determination of the contents of total flavonoids in plants.
【Fund】: 河南省科技厅国际合作项目(08430051002)资助
【CateGory Index】: O657.3;R284
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