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Determination of Tetracycline Type Antibiotics by Molybdenum and Europium Complex

Lu Jixin ,Li Juan (Department of Pharmacy,Tianjin Medical University,Tianjin 300203), Zhang Guizhu ,Sun Ying,Wang Yuemei,He Xiwen and Shi Huiming (Department of Chemsitry,Nankai University,Tianjin 300071)  
Based on the complex reactions of tetracycline with molybdenum (and Oxytetracyclinum and europium),a new method for the determination of tetracycline (oxytetracyclinum) has been developed by using molybdenum,europium complex method.Beer's law is obeyed for tetracycline (oxetracyclinum) in the range of 0.5~50μg/mL(0 6~50μg/mL) and the detection limit is 0.5μg/mL (0 6~50μg/mL).The method is rapid and simple.The method has been applied to the determination of tetracycline in urine with satisfactory results.
【Fund】: 国家自然科学基金
【CateGory Index】: TQ460.72
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