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《Journal of Radiation Research and Radiation Processing》 1987-04
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RADIATION-INDUCED DNA SINGLE STRAND BREAKS AND THEIR REJOINING IN MAMMALIAN CELLS BY HYDROXYLAPATITE CHROMATOGRAPHY

Meng Ziqiang Department of Environmental Sciences, Shanxi University, Taiyuan  
The ~(60)Co γ-ray induced single strand breaks of DNA and their rejo-ining in cultured chinese hamster lung (CHL) cells and mouse spleen cells isolatedfreshly from mice were detected by hydroxylapatite chromatography. The method isbased on the observation that the rate of denaturation of DNA to single strands inalkali is increased after irradiation, the interpretation being that this effect is due toDNA strand breaks. The percentage of labeled DNA that remained double strand after30 min alkali treatment was inversely proportional to the radiation dose within the ran-ge of 1-10Gy for the two kinds of cells. The DNA strand breaks in unirradiated spleen cells was more than in unirradia-ted CHL cells, but the yield of breaks induced per 0.01Gy was found to be similarin the spleen cells and CHL cells. In contrast with these findings, most DNA strandbreaks in the CHL cells were rejoined within 30min, the breaks were rejoined fasterin the cells incubated in medium with 20% new born calf serum after irradiation thanin medium without any serum. The repair of damaged DNA could not be detected inthe spleen cells cultured in RPMI 1640 medium without any serum.
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