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《Journal of Radiation Research and Radiation Processing》 2005-05
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Expression and localization of Smad4 protein in mouse testis after whole-body X-ray irradiation

ZHU Huaping1 ZHANG Yuanqiang1 QI Yuhong2 ZHAO Jie1 ZHAO Yong1 MA Jing1 HOU Wugang1 1 (Department of Histology and Embryology, Fourth Military University, Xi`an 710032) 2 (Department of Radiotherapy, Tangdu Hospital, the Fourth Military University, Xi`an 710032)  
The work is to determine whether and where Transforming growth factor-betas downstream Signal- ing molecule Smad4 is expressed in the testes after whole-body X-ray irradiation and shed light on the mechanisms of Transforming growth factor-betas / Smad signal pathway mediates cell fate decisions following X-ray exposure. Five groups of adult BALB / c mice, with ten mice in each group, received whole-body of X-ray at dose levels of 0.1 Gy, 0.5 Gy, 1.0 Gy, 1.5 Gy and 2.0 Gy. They were sacrificed at 16 hour, 1 week, 2 weeks, 3 weeks and 4 weeks after the irradiation. Cellular localization and expression changes were examined by immunohistochemical ABC method. Quantitative analysis of the immunostaining was made by an image analysis system. In the seminiferous tubules, the expression of Smad4 was modulated by irradiation. The immunostaining showed that 16 hour post-irradiation, there was a significant decline in the Leydig cell, and it was dose and time depended. In addition, the immunolocalization showed that Smad4 was not exclusively localized in the cytoplasm of Leydig cells, but also localized in various Stages of spermatogenesis after the exposure, especially in premeiotic spermatogonia and primary spermatocytes. There was just a little expression in the 2.0 Gy group 16 h after the irradiation and the 1.0 Gy and 1.5 Gy groups at 2 weeks after the irradiation. Therefore in the 0.1 Gy to 2.0 Gy groups at 3weeks after the irradiation, the immunostain- ing positive cells were significantly increased in spermatogonia and primary spermatocytes. There was no significant change in sertoli cells with different doses and different times after the exposure. The different expression patterns and change by dose and time of Smad4, suggest that TGF-β / smad signal pathway may affect aspect after X-ray im- pairment and Smad4 may play an important role during these periods.
【Fund】: 国家自然科学基金(30370750)资助
【CateGory Index】: R811.5;
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