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《Journal of Radiation Research and Radiation Processing》 2011-05
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Effect of electromagnetic pulses on the expression of occludin in testis of mice

XU Kai1 HOU Wugang1 ZENG Lihua2 ZHAO Jie1 LI Zhen1 ZHANG Yuanqiang1 1(Department of Histology and Embryology,Fourth Military Medical University,Xi’an 710032,China) 2(Department of Radiation Medicine,Fourth Military Medical University,Xi’an 710032,China)  
The expression and significance of blood-testis-barriar tight junction protein as well as Occludin in the testis of mice was investigated after whole-body exposure to electromagnetic pulse(EMP).Thirty adult Balb/c mice were irradiated to the whole body with 400kV/m EMP and 200 repeat while other thirty adult Balb/c mice were sham irradiated in the same condition.Specimens were harvested to prepare paraffin-embedded slice or to extract tis-sue protein and mRNA on 1,7,14,21 and 28 days after irradiation.Changes in cellular localization and expression of Occludin were examined by Real-time PCR,Western-blotting and immuno-fluorescence method,respectively.The results showed that the mRNA and protein levels of Ocludin expression were significantly decreased on 1,7 and 14 days after irradiation compared with control groups.These results indicate that EMP can reduce the expression of blood-testis-barrier tight junction protein Occludin and induce the damage of blood-testis-barrier.
【Fund】: 国家自然科学基金(No.30771138 No.30871309)资助
【CateGory Index】: Q691
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 HOUWugang ZHAO Jie ZHANG Yuanqiang ZENG Lihua GUOGuozhen (Department of Histology and Embryology, Fourth Military Medical University, Xi 'an 710032) (Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi'an 710032);Radiation effects of electromagnetic pulses on mouse blood-testis barrier[J];Journal of Radiation Research and Radiation Processing;2005-06
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Zhi-cheng,LIU Shu-chun,LI Peng-wu,KANG Shun-ai,LIANG Shuo,ZHAO Gang,GONG Shou-liang(MH Radiobiology Research Unit,School of Public Health,Jilin University,Changchun 130021,China);Changes of mitochondrial structure,ATPase and Ca~(2+) concentration in spermatogenic cells of mouse testes induced by low dose radiation[J];Journal of Jilin University(Medicine Edition);2009-04
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4 JI Xinxin HOU Wugang ZHAO Jie ZHAO Yong LI Wei ZHANG Yuanqiang (Department of Histology and Embryology, Fourth Military Medical University, Xi’an 710032);Expression and localization of Smad4, Smad1 and Smad5 in testis of mice exposed to electromagnetic pulses[J];Journal of Radiation Research and Radiation Processing;2006-06
5 JI Xinxin HOU Wugang ZHAO Jie ZHAO Yong LI Wei ZHANG Yuanqiang (Department of Histology and Embryology, Fourth Military Medical University, Xi’an 710032);The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse[J];Journal of Radiation Research and Radiation Processing;2007-02
6 XIA Hongjie~(1,2) WANG Dewen~2 1 (Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China) 2 (Department of Integrated Traditional Chinese Medicine and Western Medicine for Rehabilitation,General Hospital of Chinese Peoples Armed Police Force,Beijing 100039,China);Effects of electromagnetic radiation on organism[J];Journal of Radiation Research and Radiation Processing;2008-05
7 YANG Yang1 HOU Wugang2 ZENG Lihua3 ZHAO Jie2 LI Zhen2 ZHANG Yuanqiang21(Department of Dentistry and Clinical Medicine;Fourth Military Medical University,Xi’an 710032,China) 2(Department of Histology and Embryology,Fourth Military Medical University,Xi’an 710032,China) 3(Department of Radiation Medicine,Fourth Military Medical University,Xi’an 710032,China);The effects of electromagnetic pulses on the expression of TGF-βs in testis of mice[J];Journal of Radiation Research and Radiation Processing;2011-01
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【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 WANG Qi 1, REN Dong-qing 1, KUANG Fang 2, SUN Jing-min 2, WANG Jin 1, PANG Yi-bing 1, GUO Yao 1, GUO Guo-zheng 1. 1.Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China; 2.Institute of Neuroscience, Fourth Military Medical University, Xi'an 710032, China;The study of dose-response relationship of pulsed electromagnetic radiation on rat blood-brain-barrier[J];Chinese Journal of Disease Control and Prevention;2003-05
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