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Study the Influence of Morinda Officinalis How on the Differentiation from Marrow Stroma Cell to Osteoblast

WANG He ming,WANG Li,LI Nan(Orthopaedic and Traumatology department of FCTCM,Fuzhou,Fujian 350003, China)  
To study the influence of Morinda officinalis How on the differentiation from marrow stroma cell (MSC) to osteoblast,culture MSC in vitro by medicated blood serum method.Using 6 groups:group A(aqueous extract),group B(alcohol extract),group c(contrast),group D(classical osteogenic induction),group E(apueous extract+classical osteogenic induction),group F(alcohol extract+classical osteogenic induction),and observe ALP staining,AIR staining,the specific activity of ALP and BGP content of each group.The result showed that the order of intensity of ALP staining and AIR staining was group Fgroup Egroup Dgroup Bgroup A,and group C was negative,while the result of the specific activity of ALP and content of BGP was:group A,B,D,E,F higher than group cat the same time point(P0.05);Group E,F were higher than group D at the same time point(P0.05).It was manifested that aqueous extract and alcohol extract of Morinda officinalis How could both promote the differentiation from MSC to osteoblast,and the effect of alcohol extract was stronger than that of aqueous extract.
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