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《Journal of Forensic Medicine》 2006-03
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Forensic Application of 9 Y-STRs Fluorescence-labeled Multiplex Amplification System

SHI Mei-sen1, LI Ying-bi2, DENG Jian-qiang3, JI Qiang4, YU Xiao-jun1, HOU Yi-ping2(1.Department of Forensic Medicine, Shantou University Medical College, Shantou 515031,China; 2.West China Preclinial and Forensic College,Sichuan University,Chengdu 610041,China; 3.Institute of Forensic Sciences, Ministry of Justice, P.R.China, Shanghai 200063, China; 4.Department of Forensic Medicine, Nangjing Medical University,Nangjing 210029,China)  
Objective In order to increase significantly the discriminatory potential of Y-STR systems available to the forensic community, we have developed a system capable of simultaneously amplifying 9 Y-STR loci by fluorescence-labeled multiplex PCR technique. Methods Primers of STR loci DYS434, GATA-A10, DYS438 and DYS439 were labeled with 6-FAM, primers of STR loci DYS531, DYS557, DYS448 were labeled with HEX, and primers of STR loci DYS456, DYS444 were labeled with TAMRA, respectively. PCR products were analyzed using capillary electrophoresis and GeneScan Software on the ABI Prism310 DNA Analyzer. Series experiments were carried out to evaluate the useful value in forensic application such as the sensitivity, male specificity and genotyping DNA different tissues of the same individual. Results 9 Y-STR loci were exactly determined following optimization of the polymerase chain reaction. In a sample of 120 males, a total of 105 different haplotypes was identified, 97 of them being unique. Overall, haplotype diversity was 0.996 8. It was proved that genotyping of these 9 Y-STR loci in some sexual crime should be prior to that of automosomal STR. Conclusion The results suggest that the newly constructed 9-plex will be very powerful for establishing Y-STR database, population genetic studies and mixture stains identification.
【Fund】: 国家自然科学基金资助项目(30271446);; 高校博士点专项科研基金项目(20020610044)
【CateGory Index】: D919
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【References】
Chinese Journal Full-text Database 2 Hits
1 HUANG Yan-Mei1,2, QI Ying-Jie2, ZHU Yun-Liang1, 3, TONG Da-Yue1, WU Xin-Yao1 1. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China; 2. Department of Forensic Medicine, Xinxiang Medical University2, Xinxiang 453000, China; 3. Department of Forensic Medicine, Zhengzhou University, Zhengzhou 450002, China;Polymorphism of 7 Y-STR loci in Chinese populations by multiplex PCR genotyping using fluorescein-labeled primers[J];Hereditas;2007-10
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1 CHEN Shuai-feng1, YUAN Li2, YE Jian3. 1.Chinese pepole's public security university Beijing 100038; 2.Beijing Institute of Forensic Medicine & Science Beijing 100040; 3.Institute of Forensic Science Ministry of Public Security People's Republic of China Beijing 100038;The research development in Y chromosome STR loci.[J];The Journal of Law and Medicine;2005-01
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Chinese Journal Full-text Database 3 Hits
1 JIANG Pan-pan, ZHANG Shou-chun, YU Ning, XUAN Zhi-xing (1College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China; 2Institute of Animal Hus-bandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161,China);Research progress on sex-chromosome STR loci in parentage test[J];Chinese Bulletin of Life Sciences;2010-05
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1 HUANG Yan-mei~1,QI Ying-jie~1,YANG Bao-sheng~1,TONG Da-yue~2,WU Xin-yao~2(1.Department of Forensic Medicine,Xinxiang Medical College,Xinxiang,Henan 453000; 2.Department of Forensic medicine,Zhongshan Medical College Sun Yat-sen University,Guangzhou 510080);Genetic Polymorphism of 7 Y-STR Loci in the Yunnan Bai and Xinjiang Uygur Peoples of China[J];Acta Anthropologica Sinica;2010-01
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