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《Journal of Fuzhou University(Natural Sciences Edtion)》 2000-06
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One-step thiophilic adsorption chromatography purification of chicken immunoglobulin from egg yolk

FU Rong 1, LI Long 1, WANG Zhong-lai 1, LIN Xiang-yang 1, LIN Xiu-mei 2, RAO Ping-fan 1 (1.Institute of Bioengineering, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fujian Provincial Hospital for Maternity and Child Care, Fuzhou, Fujian 350  
This paper described the separation and purification of chicken IgY by using thiophilic adsorption chromatography (TAC). The lipoprotein was removed by gravity settling followed by filtration. The IgY with TAC was found to be homogeneous by SDS-PAGE. The biological activity of IgY and the recovery of total protein were more than 70 % and 91 %, respectively.
【Fund】: 福建省计委攻关项目!(QG98- 4 )
【CateGory Index】: TQ93
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【Co-citations】
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