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《Journal of Fuzhou University(Natural Sciences Edtion)》 2003-01
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Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus

CHENG Ji1, HONG Wen-rong1, SU Jian-zhang1, YE Li-rong2 (1. College of Qiaoxing Light Industry, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fuzhou Antibiotic General Factory, Fuzhou, Fujian 350002, China)  
The protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus was studied. The spores were cultivated in seed media firstly for 24?h, then transferred into mycelia media with 0.5%Gly and cultivated further for 24?h, that high quantity and quality mycelia which were easy to prepare protoplasts can be gotten. The experiment showed that high qulity S.tene protoplasts can be gained in 10?mg/mL lysozyme for 60?min and high qulity S.kana protoplasts can be gained in 5?mg/mL lysozyme for 75?min. By inactivating S.kana protoplasts and then fusing the two kinds protoplasts, there were three kinds recombinants colonies which were quite different in size, color and morphorlogy can be observed in plate.From these different colony, 44 positive recombinants were selected of which the highest tobramycin potency increased 39% than that of the parent strain.
【CateGory Index】: R915
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