Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fuzhou University(Natural Sciences Edtion)》 2004-02
Add to Favorite Get Latest Update

Analysis on the activity of 5α-reductase using high performance liquid chromatography

OU Min-rui~1, DAI Xiao-fu~1, XUE Feng-chun~1, ZHANG Yi~2, XU Xiao-ping~1 (1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Office of Educational Administration, Fuzhou University, Fuzhou, Fujian 350002, China)  
An convenient and quick analytical method has been established for the determination of 5α-reductase activity using high performance liquid chromatography(HPLC). This method is suitable for high throughput screening(HTS) of 5α-reductase inhibitor in vitro. The stainless steel column was 300?mm×4.6?mm (i.d.) packed with totally porous, spherical carbon particles. A solution of methanol and water(70∶30, V/V) was employed as the mobile phase. The flow rate was 0.5?mL·min~(-1). Chromatography was performed with ultra violet detector at 242 nm, column temperature at 32?℃. Linear calibration curve for T was measured within the range of 6-300 ng/?mL with correlation coefficient of 0.999?2. The lowest detection limit was 3?ng.
【Key Words】: HPLC 5α-reductase activity detection HTS
【Fund】: 福州大学本科生科研实践资助项目(02062)
【CateGory Index】: R446.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 JI Bao-quan,YANG Ai-gang,ZHU Yong-ning,PANG Yue,TANG Ling,FENG Bao-min,WANG Yong-qi(College of Bio-engineering,Dalian University,Dalian 116622,China);Study the 5α-reductase Inhibitory Activity of Urtica fissa E. Pritz's Extract[J];Lishizhen Medicine and Materia Medica Research;2009-08
【Citations】
Chinese Journal Full-text Database 1 Hits
1 CUI Yugui,ZHANG Guiyuan Department of Reproductive Endocrinology,National Research Institute for Family Planning,Beijing 100081;The steroid 5a-reductase isozyme activities in foreskin of Chinese adult men[J];JOURNAL OF REPRODUCTIVE MEDICINE;1995-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Na,CHU Yan-liang,LI Jing,WANG Kai-xuan(College of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212018);Dynamic Analysis on Extraction Technique and Four Seasons Content of Leaf Protein Concentrate from Camphor Trees[J];Northern Horticulture;2011-07
2 CHEN Jie-bo,XU Xiao-ping,OU Min-rui,XIE Jun (College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou,Fujian 350002,China);Screen steroid 5 alpha-reductase inhibitors in vitro by high performance liquid chromatography[J];Journal of Fuzhou University(Natural Sciences Edtion);2005-05
3 Jiang Xuebin,Chen Yingheng,Feng Yanping,Lei Dezhu (College of Life Science,Guangzhou University,Guangzhou 510006,China);Effect of Disruption Condition on SOD Activity of Bacillus cereus[J];Guangdong Chemical Industry;2010-05
4 CUI Yu-gui,HUA Li-xin,WANG Xing-hai,MA Ding-zhi,JIA Yue,CAI Rui-fen,TONG Jian-sun,YANG Ming-de,GAO Li. Reproductive Medicine Center of the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China (CUI Yu-gui,JIA Yue,YANG Ming-de,GAO Li); Urological Department of the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China (HUA Li-xin); Jiangsu Provincial Institute for Family Planning,Nanjing 210036,China (WANG Xing-hai,MA Ding-zhi,CAI Rui-fen,TONG Jian-sun);Study on the Androgen Level and 5α-reductase Activity in Prostate Tissues of Dogs With Benign Prostatic Hyperplasia[J];Journal of International Reproductive Health/Family Planning;2009-05
5 Ren Liyun 1 Huang Kejian 2 Qin Aizhi 1 Guo Yongan 3 Pan Zhiwen 4 Mou Kejian 1 Liu Yusheng 1 1 College of Agriculture, Guangxi University, Nanning, 530005; 2 Institution of Forensic Science,Public Security of Guangxi Province, Nanning, 530012; 3 College of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004; 4 Liuzhou Product Quality Supervision Testing In- stitution, Liuzhou, 545001;Effect of Ingredients and Physics Structure of Towel Gourd Leaves on Feeding and Orientation of Aulacophora lewisii[J];Genomics and Applied Biology;2009-05
6 DING Wen-Jie, YUE Si-jun, GUO Wei, QIN Shao-yan, JIA Shi-ru(Tianjin University of Science and Technology, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin 300457, China);Comparison of Different Cell Disruption Methods for Nostoc Flagelliforme[J];Modern Food Science and Technology;2009-11
7 ZHANG Ying-long et al(Institute of Bioengineering and Technology,Inner Mongolia University of Science and Technology,Baotou,Inner Mongolia 014010);Screening of Ethanol-producing Yeasts by Xylose Fermentation[J];Journal of Anhui Agricultural Sciences;2011-18
8 ZHENG Hua-yan1,LI Jin-cheng2,SUN Xin1, QUE Guang-long1,XU Dan-dan1,ZHANG Fu-yan1(1.School of Environmental Science and Engineering,Guilin University of Technology,Guilin 541004,China; 2.Guangxi Provincial Key Lab of Environmental Engineering and Environmental Protection Assessment,Guilin 541004,China);Treatment of Frigon-Manufacturing Wastewater Using a Bench-scaled Anaerobic Baffled Reactor[J];Environmental Science & Technology;2011-09
9 OU Min rui 1,DAI Xiao fu 1,ZHOU Xun sheng 2,CHEN Zan qiu,XU Xiao ping(1.College of Chemistry and Chemical Engineering; 2. Office of Educational administration, Fuzhou University, Fuzhou, Fujian 350002, China);Summarization on the Study of 5α-reductase and its Inhibitors[J];Strait Pharmaceutical Journal;2003-06
10 WANG Hai-ou,GAO Jing,JIANG Yan-jun,ZHOU Li-ya ( School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China );Optimization of Papain Immobilization by Sol-Gel Method with Hybrid Precursors[J];Journal of Hebei University of Technology;2010-02
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Yong-qi,FENG Bao-min,LI Fan,LIU Jing-yan,YANG Ai-gang,PANG Yue,MU Hong-mei(Drug Researching Institute,Dalian University,Dalian 116622,China);Review on the research of BPH treatment by urtica plants[J];Journal of Dalian University;2005-02
2 CHEN Jie-bo,XU Xiao-ping,OU Min-rui,XIE Jun (College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou,Fujian 350002,China);Screen steroid 5 alpha-reductase inhibitors in vitro by high performance liquid chromatography[J];Journal of Fuzhou University(Natural Sciences Edtion);2005-05
3 WU SHu-Fang TU Zeng-Hong (State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031);Pharmacology and Toxicology Study of Epristeride: A Novel Drug against Benign Prostate Hyperplasia[J];The Chinese Journal of Clinical Pharmacology;2000-06
4 RUI Caixia,YANG Xiaoming Beijing Institute of Radiation Medicine, Beijing, 100850, China;Association of Steroid 5α-reductase with Hormone-associated Tumour[J];Journal of Medical Molecular Biology;2006-04
5 OU Min rui 1,DAI Xiao fu 1,ZHOU Xun sheng 2,CHEN Zan qiu,XU Xiao ping(1.College of Chemistry and Chemical Engineering; 2. Office of Educational administration, Fuzhou University, Fuzhou, Fujian 350002, China);Summarization on the Study of 5α-reductase and its Inhibitors[J];Strait Pharmaceutical Journal;2003-06
6 Liu, Yun(The Third Pharmaceulical Works of Beijing,Beijing,100081) Han,Guang-Dian(Institule of Materia Media,Chinese Acadewmy of Medical Sciences,Beijing,100050);Recent Progress of Synthetic Methods for Corticoid Side Chain[J];CHINESE JOURNAL OF SYNTHETIC CHEMISTRY;1994-03
7 Wang Pu 1,2 ,Chen Xiyang 2,Yu Bingjun 2,Cen Peilin 1 (1 Institute of Biochemical Engineering,Zhejiang University,Hangzhou,310027; 2 College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou,310032 );Applications of New Techniques on Steroid Synthesis by Microbial Transformation[J];Chemical Industry and Engineering Progress;2002-11
8 CHE Cheng-bin, LIU Jing-chun, WU Bao-hua (Applied Sciences College, Harbin Univ. Sci. Tech., Harbin 150080, China; 2.Harbin Institute of Food, Harbin 150000, China);Microbial Transformation of Soybean Sterol[J];Journal of Harbin University of Science and Technology;2002-01
9 YU Kai(Bioengineer College of Southwest Jiaotong University,Chengdu 610031,China);Inhibition of Pterocarpus indicus Willd extract on 5α-reductase[J];West China Journal of Pharmaceutical Sciences;2007-05
10 WU Xue-yan,MAO Jiang-feng,SHI Yi-fan,DENG Jie-ying (Peiking Union Medical College,department of endocrinology in Peiking Union Hospital,100730,China);Steroid 5α-reductase and 5α-reductase type 2 deficiency[J];Basic & Clinical Medicine;2006-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 WANG Xinxiang1,WU Ya2,3,TAO Yan2,TAO Jingchao21.Henan Research Institute of Chemistry and Engineering Co.Ltd.,Zhengzhou 450052,China;2.Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China;3.School of Pharmacy,Henan College of Traditional Chinese Medicine,Zhengzhou 450008,China;Synthesis and characterization of novel D-ring modified dehydroepiandrosterone derivatives[J];Journal of Tianjin Normal University(Natural Science Edition);2011-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 CUI Yugui,ZHANG Guiyuan Department of Reproductive Endocrinology,National Research Institute for Family Planning,Beijing 100081;The steroid 5a-reductase isozyme activities in foreskin of Chinese adult men[J];JOURNAL OF REPRODUCTIVE MEDICINE;1995-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved