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《Journal of Fuzhou University(Natural Sciences Edtion)》 2005-05
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Screen steroid 5 alpha-reductase inhibitors in vitro by high performance liquid chromatography

CHEN Jie-bo,XU Xiao-ping,OU Min-rui,XIE Jun (College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou,Fujian 350002,China)  
An in vitro method to screen steroid 5 alpha-reductase inhibitors by high performance liquid chromatography was reported.Steroid 5 alpha-reductase,series concentration of testosterone and Finasteride(or Epristeride) were incubated at 37?℃ for 30?min.Then the samples were analyzed by HPLC with C18 column.The descending rate of the concentration of testosterone showed the activity of the enzymatic.And inhibition constant(K_i) of the steroid 5α-reductatse inhibitors could be calculated according to the Lineweaver-Burk plots.The K_i values of Finasteride and Epristeride were K_i=4.32±3.8?μmol/L and 1.14±0.23?μmol/L,respectively.The results indicated that the method is rapid and efficient to screen steroid 5 alphareductase inhibitors in vitro.
【Key Words】: high performance liquid chromatography(HPLC) steroid 5α-reductatse inhibitors Finasteride Epristeride
【CateGory Index】: O657.72;TQ464;
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【Co-citations】
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