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Structure investigation of water soluble polysaccharide H_n isolated from the fruits of Hippophae rhamnosides L

CHEN Yu xiang 1,ZHANG Li ping 2, LIANG Zhong yan 2, MIAO Chun yan 2, ZHANG Yi shen 2 (1 Jilin University of Technology Bilolgy Resource Enginecring, Changchun 130025, China; 2 College of Life Science, Northeast Normal University, Changchun  
The crude polysaccharide isolated from the fruits of Hippophae rhamnosides L . Serveral fractions were got after fractionation. We have studied the structure of H n . Its homogeneity was confirmed by glass fiber electrophoresis,chromatography on sepharose CL-4B and optical rotation measurement. Its molecular weight estimated by gel filtration is approximately 100 000. By means of IR, GC, GC-MS, 13 C-NMR, periodate oxidation, Smith degradation, methylation analysis, partical hydrolysis with acid and amylase hydrolysis ect. The structure of H n was identified. Its basic structure is composed of (1→4)-linked glucose,(1→6)-linked mannose and (1→6)-linked galactose residues in main chain ;and (1→)-linked glucose,(1→)-linked arabinose, (1→6)-linked galactose and (1→4)-linked glucose in side chains. Side chains attached to O—3 of glucose, mannose and galactose. On average, among 11 main chain residues, there are 5 branches.
【Fund】: 国家自然科学基金!资助项目 ( 93 9870 12 )
【CateGory Index】: Q539
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