Comparisons of Genetic Analysis Based on ISSR and SSR in PGMS and TGMS Rice Lines
Li Jinbo 2,3 Jiang Liangrong 3 Li Chunhai 1 Mou tongmin 1* 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070 2 Hybrid Rice Research and Development Center, Hubei Academy of Agricultural Sciences, Wuhan, 430070 3 Hainan Provincial Key Laboratory of Crop Molecular Breeding, Sanya, 572025
In this study, DNA fingerprints of 24 elite photoperiod sensitive genic male sterile (PGMS) and thermo sensitive genic male sterile (TGMS ) rice lines were established using inter simple sequence repeats (ISSR) markers and simple sequence repeats (SSR) markers. 13 ISSR primers could amplify 174 polymorphic bands and 20 SSR primers could amplify 62 polymorphic bands. The numbers of average polymorphic bands produced by per ISSR primer are 13 38 more than those produced by per pair of SSR primer. The results showed that clustering analysis based on ISSR data is very similar to that based on SSR data. These 24 sterile lines used in this research were divided into three groups, indica group, indica like and japonica group. In the indicagroup, 19 sterile lines were also clustered into three subgroups. 7 sterile lines derived from Annong S 1 were clustered together, which show obviously genetic differences compared to 11 indica sterile lines derived from Nongken 58S. There are a little differences in genetic distance and genetic relationship of tested sterile lines calculated by depending on data from ISSR or SSR. The results of this research demonstrated that it is more suitable for using ISSR and SSR as marker to do DNA fingerprint construction, classification and genetic analysis.