Pyramiding CMS Fertility Restoring Gene Rf_1 and Transgene Bt with Molecular Markerassisted Selection in Gossypium Hirsutum L
Liu Liwang Zhu Xiefei Guo Wangzhen Zhang Tianzhen * National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, P.R.China
BC 2 population was produced with CMS restoring line 0 613 2R and transgenic Bt line R019 as recurrent parent. Three SSR markers tightly linked to CMS restoring gene Rf 1 were used to detect the restoring gene; and Bt primers that align to Bt gene were also used to analyze Bt gene to develop transgenic Bt fertility restoring lines pyramided Rf 1 and Bt genes. Out of 59 random selected individuals in BC 2, 55 have the Rf 1 genotype of heterogeneity (Rf 1 rf); while existence rate of Bt gene is 54/59 in the materials. From the self progeny of the pyramided individuals, 10 individuals that contain both Rf 1 (Rf 1Rf 1) and Bt gene were obtained with marker aided selection, which may facilitate the breeding for elite insect resistance restoring lines and hybrid cotton.