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《Molecular Plant Breeding》 2003-04
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Saturating a Molecular Linkage Map of Rice with SSR Markers

Duan Yuanlin 1* Zhao Shouhuan 1* Wu Weiren 1,2** Zhou Yuanchang 1 Qi Jianmin 1 Pan Runsheng 1 Lin Lihui 1 Chen Zhiwei 1 Guan Huazhong 1 Mao Damei 1 1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002 2 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029 * These authors contributed equally to this work ** Corresponding author, zjweiren@pub5 fz fj cn  
We had constructed an RFLP marker lingkage map of rice based on a doubled haploid (DH) population derived from a cross between an indica rice variety Gui-630 and a japonica rice variety Taiwanjing. The map contains 175 RFLP markers and covers a total length of 1224 6cM with an average distance of 7 0cM between adjacent markers. But markers on the map are not evenly distributed and there are two breaking points on chromosomes 4 and 8. This study aimed to avoid the two breaking points and make markers distributed denser and more evenly in the map by adding some SSR markers. A total of 361 pairs of SSR primers were used to screen polymorphic markers between the parents. 183 pairs of the primers showed polymorphism and 57 SSR markers were added into the map. The new map covers a total length of 1811 2cM, 47 9% longer than the original map; the average distance between adjacent markers is 7 8cM, similar to the original map. However, the two broken points still exist, implying that the two regions might be less polymorphic and of high recombination frequency.
【CateGory Index】: S511
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