Production of Human Insulin in Transgenic Tomato
Scholar: Chen xi Supervisor: Prof. Lin Z P Peking University, Beijing, 100871
It has been suggested that oral administration of human insulin might prevent or delay the onset of the type I diabetes mellitus by suppressing autoimmune destruction. In this project, we use tomato fruit as a bioreactor to produce human insulin and test its effect as edible vaccine against diabetes. In order to enhance its expression level and natural folding, the B chain and A chain of human insulin were encoded with plant bias codons and linked with a 6 amino acids linker, which is expected to form a typical β turn. This recombinant gene was synthesized by double chain method and fused with constitutive 35S CaMV promoter and tomato fruit specific promoter 2A12, respectively. The chimeric plant expression vectors pCAM35S Ins and pCAM2A12 Ins have been introduced into tobacco and tomato by Agrobacterium mediated transformation. The expression of the synthesized insulin gene and its antibody binding activity were identified by PCR procedure and western dot blot analysis in transgenic tobacco. Further studies will focus on animal test and functional analysis.
【CateGory Index】： Q789