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Genetic Diversity in Cultivated Fragrance Rice Varieties Revealed by SSR and ISSR Markers

Wang Jinhua1,2 Luo Wenyong1 Chen Jianwei1 Liu Yuanfeng1,2 Wu Qiuhua1,2 Li Xiaofang1*1 Rice Research Institute Academy of Guangdong Agricultural Sciences, Guangzhou, 510640; 2 College of Life Science, South China Normal University, Guangzhou, 510631* Corresponding author, lixiaofang38@263.net  
The objective of the present study was to make use of efficient molecular marker systems to reveal genetic diversity among 33 fragrance cultivars from 10 countries in Asia. The cultivar groups were analyzed by using 24 pairs of SSRs and 36 ISSR primers. A total of 93 and 181 polymorphic bands were found in 33 varieties respectively each kind of markers. The average number of alleles per SSR locus was 4.23 with a range from 3 to 8. The average number of polymorphism bands per ISSR primer was 5.03 with a range from 3 to 8. Genetic similarities among the 33 rice accessions ranged from 0.294 to 0.884 and from 0.595 to 0.867, respectively by means of SSR and ISSR markers. The clustering analysis based on ISSR data is very identical to that based on SSR data basically. The results were consistent with three types of climate which the cultivars come from. Consequently, SSR and ISSR markers could be used as a useful tool for measuring genetic diversity.
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