Genetic Relationship among Several Maize Inbreds Revealed by SSR Markers
Liu Xihui1,2 Liu Wenxin2 Zhang Yirong1,2 Liu Zhanxian1,2 Zhang Shukui3 Dai Jingrui1,2*1 National Maize Improvement Center, Beijing, 100094; 2 College of Agronomy and Biotechnology, China Agriclutural University, Beijing, 100094; 3 Experiment Station of Quzhou, China Agriclutural University, Handan, 100094
The genetic diversity of twelve maize (Zea mays L.)inbred lines applied widely in the past and present was analyzed with simple-sequence repeats (SSRs) and the heterotic groups were classified. One hundred and seven pairs of primers that are distributed uniformly throughout the maize genome and clear amplify the bands which have polymorphism were selected for SSR analysis. Totally 419 alleles were identified, and the average number of alleles per SSR locus was 3.9 in these lines. The mean value of the polymorphic-index content (PIC) for the SSR loci was 0.60. By clustering analysis, the twelve inbred lines were divided into 6 clusters, as follow: Ⅰ(Zong31, Zong3, Zi330, and Va35), Ⅱ(Ji63 and Lv28), Ⅲ (Huangzaosi and Daqiu36), Ⅳ(B73), Ⅴ(Tai183), and Ⅵ(87-1 and P138). The study revealed that the relationship between the clusters classified by SSRs and the pedigree records was basically corresponding. Zong31 and Zong3 derived from the same synthetics D were assigned to Ⅰcluster in the study. The relationships of Zong31 and Zong3 with their parent ancestors which consist of the synthetics D were that, both lines were closest to Zi330, next to Va35, while Ji63, Huangzaosi, Lv28, B73, Daqiu36 and Tai183 were further. Compared with their parent ancestors, both lines of Zong31 and Zong3 have greater dominance when they were crossed with Ⅵ cluster.
【CateGory Index】： S513