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《Molecular Plant Breeding》 2005-03
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Cloning of RGA Related to Stem Nematode Resistance of Sweetpotato (Ipomoea batatas (L.) Lam.) with Modified SSAP

Liu Zhesheng Liu Qingchang* Zhai Hong Wang Yuping The College of Agronomy and Biotechnology, China Agricultural University, Beijin g,100094  
A mutant, Nongda 603, resistant to sweetpotato stem nematode was obtain ed from the gamma-irradiated progenies of sweetpotato cv. Xushu 18 susceptible t o the stem nematode. Degenerate primers were designed from the conserved NBS (nu cleotide-binding site) motifs of nematode resistance genes isolated from other c rops, and used in modified SSAP (sequence-specific amplification polymorphisms) PCR amplification with the temple of Nongda 603 and Xushu 18. Two fragments (the fragments 54 and 72) with NBS conserved domain of resistance genes were amplifi ed from Nongda 603, not from Xushu 18. DNA and peptide-sequence similarity and h omology between the sequence of fragments cloned and the resistance genes report ed in other crops were determined by blast analyses through GenBank. The results showed that these two sequences had low homology to the resistance genes report ed. Primers designed from the sequences of the two fragments cloned were used in PCR amplification with the templates of resistant and susceptible sweetpotato v arieties. No polymorphic band was amplified by the primers from the fragments 72 , however, polymorphic bands were obtained by the PCR with the primers from the fragment 54. Thus, it is thought that the sequence 54 is a RGA related to sweetp otato stem nematode resistance.
【CateGory Index】: S435.32
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Chinese Journal Full-text Database 6 Hits
1 Chen Ling-yun~(1,2) Diao Ying~1 Yang Xin-sun~2 Hu Zhong-li~1 (1 College of Life Sciences,Wuhan 430072;2 Food Crops Institute,Hubei Academy of Agricultural Sciences,Wuhan 430070);Application of DNA molecular markers technology in the sweetpotato breeding[J];Anhui Agricultural Science Bulletin;2006-09
2 JIA Zhao-dong et al (Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014);Research Progress of Control and Resistance Breeding for Stem Nematode Disease in Sweet Potato[J];Journal of Anhui Agricultural Sciences;2008-02
3 Hou Fuyun1 Zhao Bing2 Wang Qingmei1 Li Aixian1 Zhang Haiyan1 Liu Guozhen1 Zhang Liming1* 1 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100; 2 Jinan Nursery Garden, Jinan, 250100;Progress on Molecular Biology of Sweetpotato[J];Molecular Plant Breeding;2006-S1
4 Zhai Hong Liu Qingchang Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, Beijing Key Laboratory of Crop Genetic Improvement, Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing, 100193;Expression Analysis of Sweetpotato Myo Inositol-1-Phosphate Synthase Gene[J];Molecular Plant Breeding;2009-03
5 ZHANG Chao-fan1,2,HUANG Yan-lan1,ZHOU Hong1(1.Hunan Crop Reaearch Institute,Changsha 410125,PRC;2.Graduate School of Central South University Long Ping branch,Hunan,Changsha 410125,PRC);Application of DNA Molecular Markers in Sweetpotato Breeding[J];Hunan Agricultural Sciences;2007-06
6 TANG Jun,ZHOU Zhi-lin,ZHANG Yun-gang,et al.(Sweet Potato Research Institute of CAAS,Xuzhou Jiangsu 221121,China);Advances in Research on Germplasm Resources of Chinese Sweet potato [Ipomoea batatas(L.) Lam.][J];Journal of Shanxi Agricultural University(Natural Science Edition);2009-05
China Proceedings of conference Full-text Database 2 Hits
1 LIU Qing-chang ZHAI Hong WANG Yu-ping College of Agronomy & Biotechnology,China Agricultural University,Beijing 100094;Progress in Cell Biotechnology and Molecular Breeding of Sweetpotato[A];[C];2005
2 CAO Qing-he ZHANG An MA Dai-fu XIE Yi-ping LI Xiu-ying WANG Xin LI Peng (Institute of Sweetpotato,Chinese Academy of Agricultural Sciences,Xuzhou Sweetpotato Research Center in fiangsu Province,Xuzhou 221121,China);Selection of Wild Elite Resistant Resources of Sweetpotato and Breeding of New Types of Interspecific Hybrids[A];[C];2008
Chinese Journal Full-text Database 1 Hits
1 Sun Deling 1,Zhao Qiancheng 2,Song Wenqin 1,and Chen Ruiyang 1 ( 1Department of Biology,Nankai University,Tianjin 300192; 2Tianjin Vegetable Research Institute,Tianjin 300384);Relationships Analysis of Chinese Cabbage Species by AFLP[J];Acta Horticulturae Sinica;2001-04
Chinese Journal Full-text Database 10 Hits
1 FU Xiao-yi et al (Agriculture Academy Agriculture University in Hebei, Baoding,Hebei 071001);Signal Transduction of Plant Disease Resistance[J];Journal of Anhui Agricultural Sciences;2005-05
3 DING Cheng-long~(1,2),SHEN Yi-xin~2,GU Hong-ru~1,MIURA Yuichi~3(1.Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;3.Institute of Forage Crop,Japan Grassland Farming and Forage Seed Association,Tochigi 329-2742,Japan);Isolation and cleaved amplified polymorphic sequence markers of disease resistance gene analogues in Lolium multiflorum[J];Acta Prataculturae Sinica;2006-03
4 TANGKexuan1,KAI Guoyin1,3,ZHANGLei1,2, PANZhen2, ZHAO Lingxia1, SUN Xiaofen1, ZHENGJingui3of Life Sciences, Fudan University,Shanghai 200433,China; 2.SchoolofPharmacy,SecondMilitary Medical University,Shanghai 200433,China;3.Biotechnology Center, Fujian Agricultural and Forest University, Fuzhou 350002,China);Advancesin RACE and Its Application in Plant Gene Cloning[J];Journal of Fudan University;2002-06
5 CHEN Xuan-yang, YUAN Zhao-nian, ZHANG Zhao-juan, ZHENG Ya-feng, ZHENG Jin-gui (College of crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Isolation of total RNA from tubers of Ipomoea batatas and cloning of conserving domain sequence of psy gene[J];;2005-01
6 CHEN Xuan-yang~(1,2),LIN Shi-qiang~(1,3),YUAN Zhao-nian~2,ZHANG Zhao-juan~2,ZHENG Jin-gui~1(1.Agricultural Product Quality Institute;2.College of Crop Science;3.College of Life Science,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China);Cloning and construction of plant expression vector of zds gene from sweetpotato(Ipomoea batatas L.)[J];;2005-04
7 PAN Da-ren~1,CHEN Guan-shui~2,ZHOU Yi-fei~1,GUO Jin-ping~3,CHEN Jian-qiu~1(1.College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;3.Longyan Branch of Fujian Tobacco Corporation,Longyan,Fujian 364000,China);The primary study on clonging and partial sequence analysis of a gene for nematode resistance in sweet potato[J];;2006-01
8 Kong Wei 1 Liu Xueyi 2* 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 2 Institute of Industrial Crop, Shanxi Academy of Agricultural Science, Fenyang, 032200;Molecular evolution of plant specific resistant genes[J];Molecular Plant Breeding;2003-01
9 Xu Bingqiang Du Zhongjun Huang Junsheng Tropical Biotechnology Research Institute, China Academy of Tropical Agriculture Science, Haikou, 571101 State Key Lab for Tropical Crops Biotechnology, China Academy of Tropical Agriculture Science, Haikou, 571101 Entironment And Plant Protection Research Institute, China Academy of Tropical Agriculture Science, Danzhou, 573717;Advances in the Cloning Candidate Disease Resistant Genes with the RGA Cloning Method[J];Molecular Plant Breeding;2004-03
10 Li Chunlai Zhang Huaiyu*Life Science College, Sichuan Agricultural University, Ya'an, 625014;Research Advance of Resistance Gene Analogs in Plant[J];Molecular Plant Breeding;2004-06
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 SONG Ji-xuan et al(Biological Technology Institute of Guizhou Province,Guiyang,Guizhou 550006);Application of DNA Molecular Markers in Genetics and Breeding of Sweet Potato[J];Journal of Anhui Agricultural Sciences;2008-30
2 DENG Ping~1 LI Ren-quan~2 (1.The Shehong Middle School,Sichuan shehong 629200 2.The Sichuan Vocational and Technical College Sichnan Suining 629000);The Molecular-markers Technique and Its Applications in the Sweet Potato Germplasm Resources and the Genetic and Breeding[J];Journal of Sichuan Vocational and Technical College;2007-03
3 Zhao Baojie 1 Hou Fuyun 2 Wang Qingmei 2 Li Aixian 2 Zhang Liming 21 Plant Science and Technology College, Qindao Agricultural University, Qindao, 266109; 2 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, 250100;Cloning of Dihydroflavonol 4-reductase Gene from Sweetpotato and its Expression in E.coli[J];Molecular Plant Breeding;2008-03
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
2 Lin Weishen ( Inner Mongolia Agricultural College );A STUDY ON THE CLASSIFICATION OF CHINESE CABBAGES[J];Acta Horticulturae Sinica;1980-02
3 Cao Jiashu and Cao Shouchun(Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029; Department of Horticulture,Nanijing Agricultural University, Nanjing 210095);A Preliminary Report on Identification of Crossing in the Origin of Chinese Cabbage-heading[J];;1995-01
4 Cao Jiashu 1 , Cao Shouchun 2 , Miao Ying 1, and Lu Gang 1 ( 1 Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029; 2 Department of Horticulture,Nanjing Agricul tural University,Nanjing 210095);Cldistic[J];;1997-01
5 Tan Chi-meng (Department of Horticulture, Sunyang Agricultural College);THE ORIGIN, DISTRIBUTION AND EVOLUTION OF THE CULTIVARS OF CHINESE HEAD CABBAGE(B. COMPESTRIS SSP. PEKINENSIS)[J];Scientia Agricultura Sinica;1979-04
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