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《Molecular Plant Breeding》 2005-03
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Identification of Chinese Cabbage Cultivars by AFLP Molecular Markers

Song Shunhua Zheng Xiaoying* Beijing Vegetable Research Center, Beijing, 100089  
In this study, AFLP was used to evaluate the genetic polymorphism of Ch inese cabbages, including 90 cultivars collected from 7 different culture region s. 20 pairs of AFLP primers were screened. The different pairs of primer differs widely in their ability to detected genetic polymorphism of Chinese cabbage. Th e range of polymorphic bands detected by individual pair of primer were from 9 t o 32. E-ACA/M-CTG which detected 71 fragments has particularly high efficiency d etecting 32 polymorphic fragments (45.7%), which was able to distinguish all of the 90 accessions. E-ACA/MCTG was also used to detected two hybrid cultivars (Be ijingxin 2 and Jingxiawang) of Chinese cabbage, respectly 10 plants. AFLP marker s in different plant of same cultivars were accordance, but one of the Beijingxi n 2 differs from others. The result showed that AFLP markers can be used to stud y Chinese cabbage fingerprinting, identify and distinguish cultivars.
【CateGory Index】: S634.1
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