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《Molecular Plant Breeding》 2005-03
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Establishment of a New Determination Method for the Pigment of Natural Colorful Cotton

Zhan Shaohua1,2 Lin Yi1* 1 Life Science School, Anhui Agricultural University, Hefei, 230036; 2 Biology D epartment, Wanxi University, Liu'an, 237012  
The method for purifying the pigment of natural colorful cotton was imp roved at first. On this basis, the following spectra were determined or quantita tive relationship was analyzed: ①The UV spectra of the H2O solution of the puri fied pigment. ②The visible spectrum of the pH10.0 NH3-NH4Cl buffer solution of the purified pigment and the quantitative relationship between values of optical density at 510nm and its relative concentration of the purified pigment. ③The quantitative relationship between values of optical density of the unpurified pi gment at 510nm and its relative concentration in the pH10.0 NH3-NH4Cl buffer sol ution. Found out:① The UV spectra of the H2O solution of the purified pigment w ere affect by pH values. ②There was a small absorption peak at 510nm in the spe ctrum of the pH10.0 H2O solution of the purified pigment. ③There was a small ab sorption peak at 510nm in the visible spectrum of the pH10.0 NH3-NH4Cl buffer so lution of the purified pigment too. ④There was a linear relationship between op tical density of the purified pigment at 510nm and its relative concentration, w ith the regression equation: y=0.033x,correlation coefficient: y=0.9983, and R2= 0.9923. ⑤There was a linear relationship between optical density of the unpurif ied pigment at 510nm and its relative concentration in the pH10.0 buffer solutio n too, with the regression equation: y=0.0395x,correlation coefficient: y=0.9898 , and R2=0.9926. So a new determination method for the unpurified pigment was set up accord ing to the UV spectrum characteristics. The new method is convenient and accurac y verified by repeated experiments and statistic test, with SD(the standard devi ation)=1.8528×10-5,CV(coefficient of variation)=1.22×10-4,and the average of o ptical density X=0.1516. The new method has a certain significance to the study on the genetic law of cotton fiber color and the breeding of natural brown cotto n?
【CateGory Index】: S562
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